{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:19:15Z","timestamp":1791188355866,"version":"4.1.0"},"reference-count":54,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2002,12,23]]},"abstract":"<jats:p>The nuclear structures that contain symmetrical dimethylated arginine (sDMA)\u2013modified proteins and the role of this posttranslational modification is unknown. Here we report that the Cajal body is a major epitope in HeLa cells for an sDMA-specific antibody and that coilin is an sDMA-containing protein as analyzed by using the sDMA-specific antibody and matrix-assisted laser desorption ionization time of flight mass spectrometry. The methylation inhibitor 5\u2032-deoxy-5\u2032-methylthioadenosine reduces the levels of coilin methylation and causes the appearance of SMN-positive gems. In cells devoid of Cajal bodies, such as primary fibroblasts, sDMA-containing proteins concentrated in speckles. Cells from a patient with spinal muscular atrophy, containing low levels of the methyl-binding protein SMN, localized sDMA-containing proteins in the nucleoplasm as a discrete granular pattern. Splicing reactions are efficiently inhibited by using the sDMA-specific antibody or by using hypomethylated nuclear extracts, showing that active spliceosomes contain sDMA polypeptides and suggesting that arginine methylation is important for efficient pre-mRNA splicing. Our findings support a model in which arginine methylation is important for the localization of coilin and SMN in Cajal bodies.<\/jats:p>","DOI":"10.1083\/jcb.200207028","type":"journal-article","created":{"date-parts":[[2002,12,23]],"date-time":"2002-12-23T14:43:29Z","timestamp":1040654609000},"page":"957-969","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":181,"title":["Symmetrical dimethylarginine methylation is required for the localization of SMN in Cajal bodies and pre-mRNA splicing"],"prefix":"10.1083","volume":"159","author":[{"given":"Franc\u0327ois-Michel","family":"Boisvert","sequence":"first","affiliation":[{"name":"1Terry Fox Molecular Oncology Group, Lady Davis Institute for Medical Research, Sir Mortimer B. Davis Jewish General Hospital, Montre\u0301al, Que\u0301bec, Canada H3T 1E2"},{"name":"2Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research, Sir Mortimer B. 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