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Mutations in chl4\/mcm17\/ctf17 segregate dicentric chromosomes through successive cell divisions without breakage, indicating that only one of the two centromeres is functional. Centromere DNA introduced into the cell is unable to promote kinetochore assembly in the absence of CHL4. In contrast, established centromeres retain their segregation capacity for greater than 25 generations after depletion of Chl4p. The persistent mitotic stability of established centromeres reveals the presence of an epigenetic component in kinetochore segregation. Furthermore, this study identifies Chl4p in the initiation and specification of a heritable chromatin state.<\/jats:p>","DOI":"10.1083\/jcb.200211116","type":"journal-article","created":{"date-parts":[[2003,3,17]],"date-time":"2003-03-17T19:06:14Z","timestamp":1047927974000},"page":"833-843","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":53,"title":["Differential kinetochore protein requirements for establishment versus propagation of centromere activity in <i>Saccharomyces cerevisiae <\/i>"],"prefix":"10.1083","volume":"160","author":[{"given":"Karthikeyan","family":"Mythreye","sequence":"first","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kerry S.","family":"Bloom","sequence":"additional","affiliation":[{"name":"Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2003,3,17]]},"reference":[{"key":"2023072213581612000_BIB1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF00331796","volume":"97","year":"1988","journal-title":"Chromosoma."},{"key":"2023072213581612000_BIB2","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0092-8674(82)90147-7","volume":"29","year":"1982","journal-title":"Cell."},{"key":"2023072213581612000_BIB3","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1242\/jcs.107.4.891","volume":"107","year":"1994","journal-title":"J. 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