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Cultured \u03b2-catenin \u2212\/\u2212 endothelial cells showed a different organization of intercellular junctions with a decrease in \u03b1-catenin in favor of desmoplakin and marked changes in actin cytoskeleton. These changes paralleled a decrease in cell\u2013cell adhesion strength and an increase in paracellular permeability. We conclude that in vivo, the absence of \u03b2-catenin significantly reduces the capacity of endothelial cells to maintain intercellular contacts. This may become more marked when the vessels are exposed to high or turbulent flow, such as at bifurcations or in the beating heart, leading to fluid leakage or hemorrhages.<\/jats:p>","DOI":"10.1083\/jcb.200212157","type":"journal-article","created":{"date-parts":[[2003,9,15]],"date-time":"2003-09-15T19:27:05Z","timestamp":1063654025000},"page":"1111-1122","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":304,"title":["The conditional inactivation of the \u03b2-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility"],"prefix":"10.1083","volume":"162","author":[{"given":"Anna","family":"Cattelino","sequence":"first","affiliation":[{"name":"1FIRC Institute of Molecular Oncology, 20139 Milan, Italy"},{"name":"2Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Liebner","sequence":"additional","affiliation":[{"name":"1FIRC Institute of Molecular Oncology, 20139 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radiosa","family":"Gallini","sequence":"additional","affiliation":[{"name":"1FIRC Institute of Molecular Oncology, 20139 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Zanetti","sequence":"additional","affiliation":[{"name":"2Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Balconi","sequence":"additional","affiliation":[{"name":"2Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Corsi","sequence":"additional","affiliation":[{"name":"3Department of Experimental Medicine and Pathology, University La Sapienza, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Bianco","sequence":"additional","affiliation":[{"name":"3Department of Experimental Medicine and Pathology, University La Sapienza, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartwig","family":"Wolburg","sequence":"additional","affiliation":[{"name":"4Institute of Pathology, University of Tu\u0308bingen, D-72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Moore","sequence":"additional","affiliation":[{"name":"5Max Planck Institute of Immunology, 79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boussadia","family":"Oreda","sequence":"additional","affiliation":[{"name":"5Max Planck Institute of Immunology, 79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Kemler","sequence":"additional","affiliation":[{"name":"5Max Planck Institute of Immunology, 79108 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Dejana","sequence":"additional","affiliation":[{"name":"1FIRC Institute of Molecular Oncology, 20139 Milan, Italy"},{"name":"2Mario Negri Institute for Pharmacological Research, 20157 Milan, Italy"},{"name":"6Department of Genetics, University of Milan, 20122 Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,9,15]]},"reference":[{"key":"2023072214320684900_BIB1","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1002\/(SICI)1097-4644(19960616)61:4<514::AID-JCB4>3.0.CO;2-R","volume":"61","year":"1996","journal-title":"J. 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