{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T17:07:57Z","timestamp":1767892077865,"version":"3.49.0"},"reference-count":45,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,11,10]]},"abstract":"<jats:p>L-selectin is a key lectin essential for leukocyte capture and rolling on vessel walls. Functional adhesion of L-selectin requires a minimal threshold of hydrodynamic shear. Using high temporal resolution videomicroscopy, we now report that L-selectin engages its ligands through exceptionally labile adhesive bonds (tethers) even below this shear threshold. These tethers share a lifetime of 4 ms on distinct physiological ligands, two orders of magnitude shorter than the lifetime of the P-selectin\u2013PSGL-1 bond. Below threshold shear, tether duration is not shortened by elevated shear stresses. However, above the shear threshold, selectin tethers undergo 14-fold stabilization by shear-driven leukocyte transport. Notably, the cytoplasmic tail of L-selectin contributes to this stabilization only above the shear threshold. These properties are not shared by P-selectin\u2013 or VLA-4\u2013mediated tethers. L-selectin tethers appear adapted to undergo rapid avidity enhancement by cellular transport, a specialized mechanism not used by any other known adhesion receptor.<\/jats:p>","DOI":"10.1083\/jcb.200303134","type":"journal-article","created":{"date-parts":[[2003,11,4]],"date-time":"2003-11-04T02:10:27Z","timestamp":1067911827000},"page":"649-659","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":46,"title":["Avidity enhancement of L-selectin bonds by flow"],"prefix":"10.1083","volume":"163","author":[{"given":"Oren","family":"Dwir","sequence":"first","affiliation":[{"name":"1Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariel","family":"Solomon","sequence":"additional","affiliation":[{"name":"1Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shmuel","family":"Mangan","sequence":"additional","affiliation":[{"name":"1Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffrey S.","family":"Kansas","sequence":"additional","affiliation":[{"name":"2Department of Microbiology-Immunology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich S.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"3Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronen","family":"Alon","sequence":"additional","affiliation":[{"name":"1Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2003,11,3]]},"reference":[{"key":"2023072905044032700_BIB1","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1038\/374539a0","volume":"374","year":"1995","journal-title":"Nature."},{"key":"2023072905044032700_BIB2","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1083\/jcb.128.6.1243","volume":"128","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072905044032700_BIB3","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1083\/jcb.138.5.1169","volume":"138","year":"1997","journal-title":"J. Cell Biol."},{"key":"2023072905044032700_BIB4","doi-asserted-by":"crossref","first-page":"11631","DOI":"10.1073\/pnas.95.20.11631","volume":"95","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072905044032700_BIB5","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1126\/science.347575","volume":"200","year":"1978","journal-title":"Science."},{"key":"2023072905044032700_BIB6","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1083\/jcb.114.2.343","volume":"114","year":"1991","journal-title":"J. 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Chem."},{"key":"2023072905044032700_BIB17","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1083\/jcb.200103042","volume":"155","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072905044032700_BIB18","doi-asserted-by":"crossref","first-page":"21130","DOI":"10.1074\/jbc.M201999200","volume":"277","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2023072905044032700_BIB19","doi-asserted-by":"crossref","first-page":"3784","DOI":"10.1073\/pnas.061324998","volume":"98","year":"2001","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072905044032700_BIB20","doi-asserted-by":"crossref","first-page":"3615","DOI":"10.4049\/jimmunol.162.6.3615","volume":"162","year":"1999","journal-title":"J. Immunol."},{"key":"2023072905044032700_BIB21","doi-asserted-by":"crossref","first-page":"13891","DOI":"10.1074\/jbc.M004939200","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072905044032700_BIB22","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1038\/379266a0","volume":"379","year":"1996","journal-title":"Nature."},{"key":"2023072905044032700_BIB23","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1083\/jcb.135.3.837","volume":"135","year":"1996","journal-title":"J. Cell Biol."},{"key":"2023072905044032700_BIB24","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/0167-5699(96)80583-4","volume":"17","year":"1996","journal-title":"Immunol. Today."},{"key":"2023072905044032700_BIB25","doi-asserted-by":"crossref","first-page":"2391","DOI":"10.1016\/S0006-3495(00)76484-8","volume":"79","year":"2000","journal-title":"Biophys. J."},{"key":"2023072905044032700_BIB26","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1093\/glycob\/10.9.849","volume":"10","year":"2000","journal-title":"Glycobiology."},{"key":"2023072905044032700_BIB27","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/S1074-7613(01)00188-1","volume":"15","year":"2001","journal-title":"Immunity."},{"key":"2023072905044032700_BIB28","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1083\/jcb.125.2.461","volume":"125","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072905044032700_BIB29","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1083\/jcb.124.4.609","volume":"124","year":"1994","journal-title":"J. Cell Biol."},{"key":"2023072905044032700_BIB30","doi-asserted-by":"crossref","first-page":"4530","DOI":"10.1073\/pnas.96.8.4530","volume":"96","year":"1999","journal-title":"Proc. Natl. Acad. Sci. 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