{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T06:56:06Z","timestamp":1792738566545,"version":"4.5.0"},"reference-count":30,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2003,8,18]]},"abstract":"<jats:p>Members of the synaptotagmin family have been proposed to function as Ca2+ sensors in membrane fusion. Syt VII is a ubiquitously expressed synaptotagmin previously implicated in plasma membrane repair and Trypanosoma cruzi invasion, events which are mediated by the Ca2+-regulated exocytosis of lysosomes. Here, we show that embryonic fibroblasts from Syt VII\u2013deficient mice are less susceptible to trypanosome invasion, and defective in lysosomal exocytosis and resealing after wounding. Examination of mutant mouse tissues revealed extensive fibrosis in the skin and skeletal muscle. Inflammatory myopathy, with muscle fiber invasion by leukocytes and endomysial collagen deposition, was associated with elevated creatine kinase release and progressive muscle weakness. Interestingly, similar to what is observed in human polymyositis\/dermatomyositis, the mice developed a strong antinuclear antibody response, characteristic of autoimmune disorders. Thus, defective plasma membrane repair in tissues under mechanical stress may favor the development of inflammatory autoimmune disease.<\/jats:p>","DOI":"10.1083\/jcb.200305131","type":"journal-article","created":{"date-parts":[[2003,8,18]],"date-time":"2003-08-18T14:05:22Z","timestamp":1061215522000},"page":"543-549","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":197,"title":["Impaired membrane resealing and autoimmune myositis in synaptotagmin VII\u2013deficient mice"],"prefix":"10.1083","volume":"162","author":[{"given":"Sabyasachi","family":"Chakrabarti","sequence":"first","affiliation":[{"name":"1Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi S.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"2Section of Immunobiology, Howard Hughes Medical Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Flavell","sequence":"additional","affiliation":[{"name":"2Section of Immunobiology, Howard Hughes Medical Institute"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn B.","family":"Marks","sequence":"additional","affiliation":[{"name":"3Department of Cell Biology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"},{"name":"4Center for Cell and Molecular Imaging, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuya","family":"Miyake","sequence":"additional","affiliation":[{"name":"6Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Liston","sequence":"additional","affiliation":[{"name":"1Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine"},{"name":"3Department of Cell Biology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly T.","family":"Fowler","sequence":"additional","affiliation":[{"name":"1Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine"},{"name":"3Department of Cell Biology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fred S.","family":"Gorelick","sequence":"additional","affiliation":[{"name":"3Department of Cell Biology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"},{"name":"5Section of Digestive Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma W.","family":"Andrews","sequence":"additional","affiliation":[{"name":"1Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine"},{"name":"3Department of Cell Biology, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2003,8,18]]},"reference":[{"key":"2023072214363793600_BIB1","doi-asserted-by":"crossref","first-page":"25427","DOI":"10.1074\/jbc.M906729199","volume":"275","year":"2000","journal-title":"J. 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