{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:19:20Z","timestamp":1790831960427,"version":"4.1.0"},"reference-count":56,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,3,15]]},"abstract":"<jats:p>The function of intestinal keratins is unknown, although keratin 8 (K8)\u2013null mice develop colitis, hyperplasia, diarrhea, and mistarget jejunal apical markers. We quantified the diarrhea in K8-null stool and examined its physiologic basis. Isolated crypt-units from K8-null and wild-type mice have similar viability. K8-null distal colon has normal tight junction permeability and paracellular transport but shows decreased short circuit current and net Na absorption associated with net Cl secretion, blunted intracellular Cl\/HCO3-dependent pH regulation, hyperproliferation and enlarged goblet cells, partial loss of the membrane-proximal markers H,K-ATPase-\u03b2 and F-actin, increased and redistributed basolateral anion exchanger AE1\/2 protein, and redistributed Na-transporter ENaC-\u03b3. Diarrhea and protein mistargeting are observed 1\u20132 d after birth while hyperproliferation\/inflammation occurs later. The AE1\/2 changes and altered intracellular pH regulation likely account, at least in part, for the ion transport defects and hyperproliferation. Therefore, colonic keratins have a novel function in regulating electrolyte transport, likely by targeting ion transporters to their cellular compartments.<\/jats:p>","DOI":"10.1083\/jcb.200308103","type":"journal-article","created":{"date-parts":[[2004,3,9]],"date-time":"2004-03-09T01:54:34Z","timestamp":1078797274000},"page":"911-921","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":113,"title":["Keratins modulate colonocyte electrolyte transport via protein mistargeting"],"prefix":"10.1083","volume":"164","author":[{"given":"Diana M.","family":"Toivola","sequence":"first","affiliation":[{"name":"1Palo Alto VA Medical Center, Palo Alto, CA 94304"},{"name":"2Stanford University School of Medicine Digestive Disease Center, Stanford, CA 94305"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selvi","family":"Krishnan","sequence":"additional","affiliation":[{"name":"4Boston Medical Center, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry J.","family":"Binder","sequence":"additional","affiliation":[{"name":"3Yale University School of Medicine, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish K.","family":"Singh","sequence":"additional","affiliation":[{"name":"4Boston Medical Center, Boston, MA 02118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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