{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:53:52Z","timestamp":1790862832073,"version":"4.1.0"},"reference-count":33,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,4,26]]},"abstract":"<jats:p>We have investigated sequential exocytosis in \u03b2 cells of intact pancreatic islets with the use of two-photon excitation imaging of a polar fluorescent tracer, sulforhodamine B, and a fusion protein comprising enhanced cyan fluorescent protein (ECFP) and the SNARE protein SNAP25 (synaptosome-associated protein of 25 kD) transfected with an adenoviral vector. Sequential exocytosis was found to account for &amp;lt;10% of exocytic events in \u03b2 cells stimulated either with glucose under various conditions or by photolysis of a caged-Ca2+ compound. Multigranular exocytosis, in which granule-to-granule fusion occurs before exocytosis, was rarely found. We detected redistribution of ECFP-SNAP25 from the plasma membrane into the membrane of the fused granule occurred in a large proportion (54%) of sequential exocytic events but in only a small fraction (5%) of solitary fusion events. Removal of cholesterol in the plasma membrane by methyl-\u03b2-cyclodextrin facilitated both redistribution of ECFP-SNAP25 and sequential exocytosis by threefold. These observations support the hypothesis that SNAP25 is a plasma membrane factor that is responsible for sequential exocytosis.<\/jats:p>","DOI":"10.1083\/jcb.200312033","type":"journal-article","created":{"date-parts":[[2004,4,26]],"date-time":"2004-04-26T19:23:16Z","timestamp":1083007396000},"page":"255-262","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":100,"title":["Sequential exocytosis of insulin granules is associated with redistribution of SNAP25"],"prefix":"10.1083","volume":"165","author":[{"given":"Noriko","family":"Takahashi","sequence":"first","affiliation":[{"name":"1Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan"},{"name":"2Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyasu","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"1Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruo","family":"Okado","sequence":"additional","affiliation":[{"name":"3Department of Molecular Physiology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Miwa","sequence":"additional","affiliation":[{"name":"3Department of Molecular Physiology, Tokyo Metropolitan Institute for Neuroscience, Fuchu, Tokyo 183-8526, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Kishimoto","sequence":"additional","affiliation":[{"name":"1Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Kojima","sequence":"additional","affiliation":[{"name":"1Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruo","family":"Abe","sequence":"additional","affiliation":[{"name":"4Department of Cellular Neurobiology, Brain Research Institute, University of Niigata, Niigata, Niigata 951-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruo","family":"Kasai","sequence":"additional","affiliation":[{"name":"1Department of Cell Physiology, National Institute for Physiological Sciences, Graduate University of Advanced Studies, Myodaiji, Okazaki 444-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2004,4,26]]},"reference":[{"key":"2023072215053671800_BIB1","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1085\/jgp.95.3.397","volume":"95","year":"1990","journal-title":"J. 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