{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:47:12Z","timestamp":1761058032198},"reference-count":59,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,8,16]]},"abstract":"<jats:p>T cell activation by nonself peptide\u2013major histocompatibility complex (MHC) antigenic complexes can be blocked by particular sequence variants in a process termed T cell receptor antagonism. The inhibition mechanism is not understood, although such variants are encountered in viral infections and may aid immune evasion. Here, we study the effect of antagonist peptides on immunological synapse formation by T cells. This cellular communication process features early integrin engagement and T cell motility arrest, referred to as the \u201cstop signal.\u201d We find that synapses formed on membranes presenting antagonist\u2013agonist complexes display reduced MHC density, which leads to reduced T cell proliferation that is not overcome by the costimulatory ligands CD48 and B7-1. Most T cells fail to arrest and crawl slowly with a dense ICAM-1 crescent at the leading edge. Similar aberrant patterns of LFA-1\/ICAM-1 engagement in live T\u2013B couples correlate with reduced calcium flux and IL-2 secretion. Hence, antagonist peptides selectively disable MHC clustering and the stop signal, whereas LFA-1 valency up-regulation occurs normally.<\/jats:p>","DOI":"10.1083\/jcb.200404059","type":"journal-article","created":{"date-parts":[[2004,8,16]],"date-time":"2004-08-16T22:48:54Z","timestamp":1092696534000},"page":"579-590","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":33,"title":["T cell receptor antagonism interferes with MHC clustering and integrin patterning during immunological synapse formation"],"prefix":"10.1083","volume":"166","author":[{"given":"Cenk","family":"Sumen","sequence":"first","affiliation":[{"name":"1Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305"},{"name":"2Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L.","family":"Dustin","sequence":"additional","affiliation":[{"name":"3Department of Pathology, New York University School of Medicine, New York, NY 10016"},{"name":"4Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark M.","family":"Davis","sequence":"additional","affiliation":[{"name":"1Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, CA 94305"},{"name":"2Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2004,8,16]]},"reference":[{"key":"2023072218201844400_BIB1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1146\/annurev.immunol.18.1.165","volume":"18","year":"2000","journal-title":"Annu. 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