{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:51:57Z","timestamp":1791294717997,"version":"4.1.0"},"reference-count":55,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,8,30]]},"abstract":"<jats:p>Listeria monocytogenes uptake by nonphagocytic cells is promoted by the bacterial invasion proteins internalin and InlB, which bind to their host receptors E-cadherin and hepatocyte growth factor receptor (HGF-R)\/Met, respectively. Here, we present evidence that plasma membrane organization in lipid domains is critical for Listeria uptake. Cholesterol depletion by methyl-\u03b2-cyclodextrin reversibly inhibited Listeria entry. Lipid raft markers, such as glycosylphosphatidylinositol-linked proteins, a myristoylated and palmitoylated peptide and the ganglioside GM1 were recruited at the bacterial entry site. We analyzed which molecular events require membrane cholesterol and found that the presence of E-cadherin in lipid domains was necessary for initial interaction with internalin to promote bacterial entry. In contrast, the initial interaction of InlB with HGF-R did not require membrane cholesterol, whereas downstream signaling leading to F-actin polymerization was cholesterol dependent. Our work, in addition to documenting for the first time the role of lipid rafts in Listeria entry, provides the first evidence that E-cadherin and HGF-R require lipid domain integrity for their full activity.<\/jats:p>","DOI":"10.1083\/jcb.200406078","type":"journal-article","created":{"date-parts":[[2004,8,30]],"date-time":"2004-08-30T18:33:10Z","timestamp":1093890790000},"page":"743-753","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":150,"title":["Role of lipid rafts in E-cadherin\u2013 and HGF-R\/Met\u2013mediated entry of <i>Listeria monocytogenes<\/i> into host cells"],"prefix":"10.1083","volume":"166","author":[{"given":"Ste\u0301phanie","family":"Seveau","sequence":"first","affiliation":[{"name":"1Unite\u0301 des Interactions Bacte\u0301ries-Cellules, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U604"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u0301le\u0300ne","family":"Bierne","sequence":"additional","affiliation":[{"name":"1Unite\u0301 des Interactions Bacte\u0301ries-Cellules, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U604"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ste\u0301phanie","family":"Giroux","sequence":"additional","affiliation":[{"name":"2Plate-forme de microscopie e\u0301lectronique, Institut Pasteur, 75015 Paris, Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Christine","family":"Pre\u0301vost","sequence":"additional","affiliation":[{"name":"2Plate-forme de microscopie e\u0301lectronique, Institut Pasteur, 75015 Paris, Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Cossart","sequence":"additional","affiliation":[{"name":"1Unite\u0301 des Interactions Bacte\u0301ries-Cellules, Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale (INSERM) U604"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2004,8,30]]},"reference":[{"key":"2023072215113288600_BIB1","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1083\/jcb.200211018","volume":"160","year":"2003","journal-title":"J. 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