{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:00:43Z","timestamp":1791273643119,"version":"4.1.0"},"reference-count":44,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,1,17]]},"abstract":"<jats:p>Contributions of actin-related proteins (Arp) 2 and 3 nucleotide state to Arp2\/3 complex function were tested using nucleotide-binding pocket (NBP) mutants in Saccharomyces cerevisiae. ATP binding by Arp2 and Arp3 was required for full Arp2\/3 complex nucleation activity in vitro. Analysis of actin dynamics and endocytosis in mutants demonstrated that nucleotide-bound Arp3 is particularly important for Arp2\/3 complex function in vivo. Severity of endocytic defects did not correlate with effects on in vitro nucleation activity, suggesting that a critical Arp2\/3 complex function during endocytosis may be structural rather than catalytic. A separate class of Arp2 and Arp3 NBP mutants suppressed phenotypes of mutants defective for actin nucleation. An Arp2 suppressor mutant increased Arp2\/3 nucleation activity. Electron microscopy of Arp2\/3 complex containing this Arp2 suppressor identified a structural change that also occurs upon Arp2\/3 activation by nucleation promoting factors. These data demonstrate the importance of Arp2 and Arp3 nucleotide binding for nucleating activity, and Arp3 nucleotide binding for maintenance of cortical actin cytoskeleton cytoarchitecture.<\/jats:p>","DOI":"10.1083\/jcb.200408177","type":"journal-article","created":{"date-parts":[[2005,1,18]],"date-time":"2005-01-18T22:58:16Z","timestamp":1106089096000},"page":"315-328","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":85,"title":["Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2\/3 complex function"],"prefix":"10.1083","volume":"168","author":[{"given":"Adam C.","family":"Martin","sequence":"first","affiliation":[{"name":"1Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Ping","family":"Xu","sequence":"additional","affiliation":[{"name":"2The Burnham Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Rouiller","sequence":"additional","affiliation":[{"name":"2The Burnham Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Kaksonen","sequence":"additional","affiliation":[{"name":"1Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yidi","family":"Sun","sequence":"additional","affiliation":[{"name":"1Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Belmont","sequence":"additional","affiliation":[{"name":"3Cytokinetics, Inc., South San Francisco, CA 94080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Volkmann","sequence":"additional","affiliation":[{"name":"2The Burnham Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorit","family":"Hanein","sequence":"additional","affiliation":[{"name":"2The Burnham Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Welch","sequence":"additional","affiliation":[{"name":"1Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Drubin","sequence":"additional","affiliation":[{"name":"1Barker Hall, Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2005,1,18]]},"reference":[{"key":"2023072215332710400_BIB1","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/35060104","volume":"3","year":"2001","journal-title":"Nat. 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