{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:44:22Z","timestamp":1790865862207,"version":"4.1.0"},"reference-count":43,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,6,20]]},"abstract":"<jats:p>Focal adhesion kinase (FAK) is a critical mediator of signal transduction by integrins and growth factor receptors in a variety of cells including endothelial cells (ECs). Here, we describe EC-specific knockout of FAK using a Cre-loxP approach. In contrast to the total FAK knockout, deletion of FAK specifically in ECs did not affect early embryonic development including normal vasculogenesis. However, in late embryogenesis, FAK deletion in the ECs led to defective angiogenesis in the embryos, yolk sac, and placenta, impaired vasculature and associated hemorrhage, edema, and developmental delay, and late embryonic lethal phenotype. Histologically, ECs and blood vessels in the mutant embryos present a disorganized, detached, and apoptotic appearance. Consistent with these phenotypes, deletion of FAK in ECs isolated from the floxed FAK mice led to reduced tubulogenesis, cell survival, proliferation, and migration in vitro. Together, these results strongly suggest a role of FAK in angiogenesis and vascular development due to its essential function in the regulation of multiple EC activities.<\/jats:p>","DOI":"10.1083\/jcb.200411155","type":"journal-article","created":{"date-parts":[[2005,6,20]],"date-time":"2005-06-20T16:45:00Z","timestamp":1119285900000},"page":"941-952","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":264,"title":["Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesis"],"prefix":"10.1083","volume":"169","author":[{"given":"Tang-Long","family":"Shen","sequence":"first","affiliation":[{"name":"1Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann Y.-J.","family":"Park","sequence":"additional","affiliation":[{"name":"1Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Alcaraz","sequence":"additional","affiliation":[{"name":"2Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Peng","sequence":"additional","affiliation":[{"name":"1Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ihnkyung","family":"Jang","sequence":"additional","affiliation":[{"name":"3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852"},{"name":"4Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pandelakis","family":"Koni","sequence":"additional","affiliation":[{"name":"5Section of Immunobiology, Yale University School of Medicine, Howard Hughes Medical Institute, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Flavell","sequence":"additional","affiliation":[{"name":"5Section of Immunobiology, Yale University School of Medicine, Howard Hughes Medical Institute, New Haven, CT 06520"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Gu","sequence":"additional","affiliation":[{"name":"3Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852"},{"name":"4Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-Lin","family":"Guan","sequence":"additional","affiliation":[{"name":"1Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2005,6,20]]},"reference":[{"key":"2023072906471206600_BIB1","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1083\/jcb.119.4.893","volume":"119","year":"1992","journal-title":"J. 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