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Actin cables that encircle the eye in the epithelial cells of the eyelid were disorganized and accumulation of filamentous actin at the umbilical ring was impaired, with loss of phosphorylation of the myosin regulatory light chain (MLC) at both sites, in ROCK-I\u2212\/\u2212 embryos. Stress fiber formation and MLC phosphorylation induced by EGF were also attenuated in primary keratinocytes from ROCK-I\u2212\/\u2212 mice. These results suggest that ROCK-I regulates closure of the eyelids and ventral body wall through organization of actomyosin bundles.<\/jats:p>","DOI":"10.1083\/jcb.200411179","type":"journal-article","created":{"date-parts":[[2005,3,7]],"date-time":"2005-03-07T20:41:04Z","timestamp":1110228064000},"page":"941-953","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":273,"title":["ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles"],"prefix":"10.1083","volume":"168","author":[{"given":"Yoshihiko","family":"Shimizu","sequence":"first","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"},{"name":"2Department of Obstetrics and Gynecology, Shiga University of Medical Science, Ohtsu, Shiga 520-2191, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean","family":"Thumkeo","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongsin","family":"Keel","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshimasa","family":"Ishizaki","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Oshima","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Oshima","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichi","family":"Noda","sequence":"additional","affiliation":[{"name":"2Department of Obstetrics and Gynecology, Shiga University of Medical Science, Ohtsu, Shiga 520-2191, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumio","family":"Matsumura","sequence":"additional","affiliation":[{"name":"3Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08855"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto M.","family":"Taketo","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuh","family":"Narumiya","sequence":"additional","affiliation":[{"name":"1Department of Pharmacology, Kyoto University Faculty of Medicine, Sakyo-ku, Kyoto 606-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2005,3,7]]},"reference":[{"key":"2023072906483990900_BIB1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1006\/excr.2000.5046","volume":"261","year":"2000","journal-title":"Exp. 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