{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:04:14Z","timestamp":1791291854374,"version":"4.1.0"},"reference-count":42,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,1,30]]},"abstract":"<jats:p>Actin is the major cytoskeletal source of dendritic spines, which are highly specialized protuberances on the neuronal surface where excitatory synaptic transmission occurs (Harris, K.M., and S.B. Kater. 1994. Annu. Rev. Neurosci. 17:341\u2013371; Yuste, R., and D.W. Tank. 1996. Neuron. 16:701\u2013716). Stimulation of excitatory synapses induces changes in spine shape via localized rearrangements of the actin cytoskeleton (Matus, A. 2000. Science. 290:754\u2013758; Nagerl, U.V., N. Eberhorn, S.B. Cambridge, and T. Bonhoeffer. 2004. Neuron. 44:759\u2013767). However, what remains elusive are the precise molecular mechanisms by which different neurotransmitter receptors forward information to the underlying actin cytoskeleton. We show that in cultured hippocampal neurons as well as in whole brain synaptosomal fractions, RhoA associates with glutamate receptors (GluRs) at the spine plasma membrane. Activation of ionotropic GluRs leads to the detachment of RhoA from these receptors and its recruitment to metabotropic GluRs. Concomitantly, this triggers a local reduction of RhoA activity, which, in turn, inactivates downstream kinase RhoA-specific kinase, resulting in restricted actin instability and dendritic spine collapse. These data provide a direct mechanistic link between neurotransmitter receptor activity and the changes in spine shape that are thought to play a crucial role in synaptic strength.<\/jats:p>","DOI":"10.1083\/jcb.200506136","type":"journal-article","created":{"date-parts":[[2006,1,31]],"date-time":"2006-01-31T01:53:53Z","timestamp":1138672433000},"page":"453-467","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":100,"title":["Localized recruitment and activation of RhoA underlies dendritic spine morphology in a glutamate receptor\u2013dependent manner"],"prefix":"10.1083","volume":"172","author":[{"given":"Vanessa","family":"Schubert","sequence":"first","affiliation":[{"name":"1Cavalieri Ottolenghi Scientific Institute, Universita Degli Studi di Torino, Azienda Ospedaliera San Luigi Gonzaga, Regione Gonzole 10, 10043 Orbassano (Torino), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Santos","family":"Da Silva","sequence":"additional","affiliation":[{"name":"1Cavalieri Ottolenghi Scientific Institute, Universita Degli Studi di Torino, Azienda Ospedaliera San Luigi Gonzaga, Regione Gonzole 10, 10043 Orbassano (Torino), Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos G.","family":"Dotti","sequence":"additional","affiliation":[{"name":"1Cavalieri Ottolenghi Scientific Institute, Universita Degli Studi di Torino, Azienda Ospedaliera San Luigi Gonzaga, Regione Gonzole 10, 10043 Orbassano (Torino), Italy"},{"name":"2Department of Human Genetics, Catholic University of Leuven, 3000 Leuven, Belgium"},{"name":"3Flanders Interuniversity Institute of Biotechnology, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2006,1,30]]},"reference":[{"key":"2023072910063444400_BIB1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.jns.2004.11.028","volume":"229-230","year":"2005","journal-title":"J. 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