{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T10:21:52Z","timestamp":1760782912654},"reference-count":58,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,10,23]]},"abstract":"<jats:p>Prevailing models place spectrin downstream of ankyrin in a pathway of assembly and function in polarized cells. We used a transgene rescue strategy in Drosophila melanogaster to test contributions of four specific functional sites in \u03b2 spectrin to its assembly and function. (1) Removal of the pleckstrin homology domain blocked polarized spectrin assembly in midgut epithelial cells and was usually lethal. (2) A point mutation in the tetramer formation site, modeled after a hereditary elliptocytosis mutation in human erythrocyte spectrin, had no detectable effect on function. (3) Replacement of repetitive segments 4\u201311 of \u03b2 spectrin with repeats 2\u20139 of \u03b1 spectrin abolished function but did not prevent polarized assembly. (4) Removal of the putative ankyrin-binding site had an unexpectedly mild phenotype with no detectable effect on spectrin targeting to the plasma membrane. The results suggest an alternate pathway in which spectrin directs ankyrin assembly and in which some important functions of spectrin are independent of ankyrin.<\/jats:p>","DOI":"10.1083\/jcb.200602095","type":"journal-article","created":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T12:45:15Z","timestamp":1161693915000},"page":"325-335","update-policy":"http:\/\/dx.doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["Spectrin functions upstream of ankyrin in a spectrin cytoskeleton assembly pathway"],"prefix":"10.1083","volume":"175","author":[{"given":"Amlan","family":"Das","sequence":"first","affiliation":[{"name":"Program in Cell & Developmental Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Base","sequence":"additional","affiliation":[{"name":"Program in Cell & Developmental Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srilakshmi","family":"Dhulipala","sequence":"additional","affiliation":[{"name":"Program in Cell & Developmental Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald R.","family":"Dubreuil","sequence":"additional","affiliation":[{"name":"Program in Cell & Developmental Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL 60607"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2006,10,23]]},"reference":[{"key":"2023072907524830300_BIB1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1038\/ncb922","volume":"5","year":"2003","journal-title":"Nat. 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Cell Biol."},{"key":"2023072907524830300_BIB12","doi-asserted-by":"crossref","first-page":"10285","DOI":"10.1073\/pnas.91.22.10285","volume":"91","year":"1994","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072907524830300_BIB13","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1002\/(SICI)1521-1878(199811)20:11<875::AID-BIES1>3.0.CO;2-P","volume":"20","year":"1998","journal-title":"Bioessays."},{"key":"2023072907524830300_BIB14","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1083\/jcb.109.5.2197","volume":"109","year":"1989","journal-title":"J. Cell Biol."},{"key":"2023072907524830300_BIB15","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1083\/jcb.149.3.647","volume":"149","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023072907524830300_BIB16","first-page":"3610","volume":"5","year":"1985","journal-title":"Mol. Cell. Biol."},{"key":"2023072907524830300_BIB17","doi-asserted-by":"crossref","first-page":"9935","DOI":"10.1021\/bi0107795","volume":"40","year":"2001","journal-title":"Biochemistry."},{"key":"2023072907524830300_BIB18","doi-asserted-by":"crossref","first-page":"18715","DOI":"10.1016\/S0021-9258(19)37020-6","volume":"267","year":"1992","journal-title":"J. Biol. Chem."},{"key":"2023072907524830300_BIB19","doi-asserted-by":"crossref","first-page":"4676","DOI":"10.1002\/j.1460-2075.1995.tb00149.x","volume":"14","year":"1995","journal-title":"EMBO J."},{"key":"2023072907524830300_BIB20","doi-asserted-by":"crossref","first-page":"27726","DOI":"10.1074\/jbc.M004959200","volume":"275","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2023072907524830300_BIB21","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1083\/jcb.200109026","volume":"155","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072907524830300_BIB22","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1083\/jcb.115.1.267","volume":"115","year":"1991","journal-title":"J. Cell Biol."},{"key":"2023072907524830300_BIB23","doi-asserted-by":"crossref","first-page":"16706","DOI":"10.1074\/jbc.M314296200","volume":"279","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2023072907524830300_BIB24","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1083\/jcb.200110003","volume":"156","year":"2002","journal-title":"J. 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Chem."},{"key":"2023072907524830300_BIB50","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1172\/JCI114792","volume":"86","year":"1990","journal-title":"J. Clin. Invest."},{"key":"2023072907524830300_BIB51","doi-asserted-by":"crossref","first-page":"23974","DOI":"10.1074\/jbc.M009307200","volume":"276","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072907524830300_BIB52","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1006\/bbrc.1995.2818","volume":"217","year":"1995","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2023072907524830300_BIB53","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1006\/bbrc.1996.1189","volume":"225","year":"1996","journal-title":"Biochem. Biophys. Res. Coomun."},{"key":"2023072907524830300_BIB54","doi-asserted-by":"crossref","first-page":"20449","DOI":"10.1016\/S0021-9258(17)30525-2","volume":"265","year":"1990","journal-title":"J. Biol. 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