{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:57:44Z","timestamp":1790866664064,"version":"4.1.0"},"reference-count":47,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,9,25]]},"abstract":"<jats:p>Apicomplexan pathogens are obligate intracellular parasites. To enter cells, they must bind with high affinity to host cell receptors and then uncouple these interactions to complete invasion. Merozoites of Plasmodium falciparum, the parasite responsible for the most dangerous form of malaria, invade erythrocytes using a family of adhesins called Duffy binding ligand-erythrocyte binding proteins (DBL-EBPs). The best-characterized P. falciparum DBL-EBP is erythrocyte binding antigen 175 (EBA-175), which binds erythrocyte surface glycophorin A. We report that EBA-175 is shed from the merozoite at around the point of invasion. Shedding occurs by proteolytic cleavage within the transmembrane domain (TMD) at a site that is conserved across the DBL-EBP family. We show that EBA-175 is cleaved by PfROM4, a rhomboid protease that localizes to the merozoite plasma membrane, but not by other rhomboids tested. Mutations within the EBA-175 TMD that abolish cleavage by PfROM4 prevent parasite growth. Our results identify a crucial role for intramembrane proteolysis in the life cycle of this pathogen.<\/jats:p>","DOI":"10.1083\/jcb.200604136","type":"journal-article","created":{"date-parts":[[2006,9,25]],"date-time":"2006-09-25T14:36:53Z","timestamp":1159195013000},"page":"1023-1033","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":159,"title":["Intramembrane proteolysis mediates shedding of a key adhesin during erythrocyte invasion by the malaria parasite"],"prefix":"10.1083","volume":"174","author":[{"given":"Rebecca A.","family":"O'Donnell","sequence":"first","affiliation":[{"name":"1Division of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Hackett","sequence":"additional","affiliation":[{"name":"1Division of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Howell","sequence":"additional","affiliation":[{"name":"2Protein Structure, National Institute for Medical Research, Mill Hill, London NW7 1AA, England, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz","family":"Treeck","sequence":"additional","affiliation":[{"name":"3Research Group Malaria II, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Struck","sequence":"additional","affiliation":[{"name":"3Research Group Malaria II, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zita","family":"Krnajski","sequence":"additional","affiliation":[{"name":"3Research Group Malaria II, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chrislaine","family":"Withers-Martinez","sequence":"additional","affiliation":[{"name":"1Division of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim W.","family":"Gilberger","sequence":"additional","affiliation":[{"name":"3Research Group Malaria II, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Blackman","sequence":"additional","affiliation":[{"name":"1Division of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2006,9,21]]},"reference":[{"key":"2023072907475559100_BIB1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S1471-4922(01)01948-1","volume":"17","year":"2001","journal-title":"Trends Parasitol."},{"key":"2023072907475559100_BIB2","first-page":"213","volume":"45","year":"1994","journal-title":"Methods Cell Biol."},{"key":"2023072907475559100_BIB3","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1084\/jem.172.1.379","volume":"172","year":"1990","journal-title":"J. Exp. Med."},{"key":"2023072907475559100_BIB4","doi-asserted-by":"crossref","first-page":"R9","DOI":"10.1186\/gb-2003-4-2-r9","volume":"4","year":"2003","journal-title":"Genome Biol."},{"key":"2023072907475559100_BIB5","doi-asserted-by":"crossref","first-page":"6229","DOI":"10.1074\/jbc.M209837200","volume":"278","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2023072907475559100_BIB6","doi-asserted-by":"crossref","first-page":"4146","DOI":"10.1073\/pnas.0407918102","volume":"102","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072907475559100_BIB7","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1111\/j.1365-2958.2005.04483.x","volume":"55","year":"2005","journal-title":"Mol. Microbiol."},{"key":"2023072907475559100_BIB8","doi-asserted-by":"crossref","first-page":"14346","DOI":"10.1074\/jbc.275.19.14346","volume":"275","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2023072907475559100_BIB9","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/S0378-1119(98)00434-X","volume":"221","year":"1998","journal-title":"Gene."},{"key":"2023072907475559100_BIB10","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1172\/JCI114753","volume":"86","year":"1990","journal-title":"J. Clin. Invest."},{"key":"2023072907475559100_BIB11","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/0166-6851(94)90134-1","volume":"64","year":"1994","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023072907475559100_BIB12","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/j.pt.2005.04.009","volume":"21","year":"2005","journal-title":"Trends Parasitol."},{"key":"2023072907475559100_BIB13","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1016\/j.ijpara.2005.04.001","volume":"35","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"2023072907475559100_BIB14","doi-asserted-by":"crossref","first-page":"4796","DOI":"10.1073\/pnas.0730883100","volume":"100","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072907475559100_BIB15","doi-asserted-by":"crossref","first-page":"10931","DOI":"10.1073\/pnas.94.20.10931","volume":"94","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072907475559100_BIB16","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1038\/nrm1334","volume":"5","year":"2004","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072907475559100_BIB17","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1083\/jcb.200301046","volume":"162","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023072907475559100_BIB18","doi-asserted-by":"crossref","first-page":"14480","DOI":"10.1074\/jbc.M211446200","volume":"278","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2023072907475559100_BIB19","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/0014-4894(83)90027-9","volume":"55","year":"1983","journal-title":"Exp. Parasitol."},{"key":"2023072907475559100_BIB20","first-page":"241","volume":"1","year":"2005","journal-title":"PLoS Pathog."},{"key":"2023072907475559100_BIB21","doi-asserted-by":"crossref","first-page":"23890","DOI":"10.1074\/jbc.M302160200","volume":"278","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2023072907475559100_BIB22","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1111\/j.1365-2958.2005.04772.x","volume":"57","year":"2005","journal-title":"Mol. Microbiol."},{"key":"2023072907475559100_BIB23","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/S0166-6851(00)00263-2","volume":"110","year":"2000","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023072907475559100_BIB24","doi-asserted-by":"crossref","first-page":"R19","DOI":"10.1186\/gb-2003-4-3-r19","volume":"4","year":"2003","journal-title":"Genome Biol."},{"key":"2023072907475559100_BIB25","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1126\/science.1087025","volume":"301","year":"2003","journal-title":"Science."},{"key":"2023072907475559100_BIB26","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0092-8674(01)00526-8","volume":"107","year":"2001","journal-title":"Cell."},{"key":"2023072907475559100_BIB27","doi-asserted-by":"crossref","first-page":"5765","DOI":"10.1074\/jbc.M308716200","volume":"279","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2023072907475559100_BIB28","first-page":"236","volume":"14","year":"2004","journal-title":"Curr. Biol."},{"key":"2023072907475559100_BIB29","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1038\/nm807","volume":"9","year":"2003","journal-title":"Nat. Med."},{"key":"2023072907475559100_BIB30","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1073\/pnas.0307318101","volume":"101","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072907475559100_BIB31","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1128\/IAI.72.1.154-158.2004","volume":"72","year":"2004","journal-title":"Infect. Immun."},{"key":"2023072907475559100_BIB32","doi-asserted-by":"crossref","first-page":"5784","DOI":"10.1128\/IAI.67.11.5784-5791.1999","volume":"67","year":"1999","journal-title":"Infect. Immun."},{"key":"2023072907475559100_BIB33","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1093\/emboj\/21.7.1577","volume":"21","year":"2002","journal-title":"EMBO J."},{"key":"2023072907475559100_BIB34","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1016\/0166-6851(90)90050-V","volume":"40","year":"1990","journal-title":"Mol. Biochem. Parasitol."},{"key":"2023072907475559100_BIB35","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.bbrc.2004.03.039","volume":"317","year":"2004","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2023072907475559100_BIB36","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1126\/science.1107449","volume":"308","year":"2005","journal-title":"Science."},{"key":"2023072907475559100_BIB37","doi-asserted-by":"crossref","first-page":"1571","DOI":"10.1084\/jem.194.11.1571","volume":"194","year":"2001","journal-title":"J. Exp. Med."},{"key":"2023072907475559100_BIB38","doi-asserted-by":"crossref","first-page":"7509","DOI":"10.1073\/pnas.97.13.7509","volume":"97","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072907475559100_BIB39","doi-asserted-by":"crossref","first-page":"40169","DOI":"10.1074\/jbc.M509631200","volume":"280","year":"2005","journal-title":"J. Biol. Chem."},{"key":"2023072907475559100_BIB40","doi-asserted-by":"crossref","first-page":"1941","DOI":"10.1126\/science.8009226","volume":"264","year":"1994","journal-title":"Science."},{"key":"2023072907475559100_BIB41","doi-asserted-by":"crossref","first-page":"1384","DOI":"10.1126\/science.1115257","volume":"309","year":"2005","journal-title":"Science."},{"key":"2023072907475559100_BIB42","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1046\/j.1365-2958.2001.02484.x","volume":"41","year":"2001","journal-title":"Mol. Microbiol."},{"key":"2023072907475559100_BIB43","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.cell.2005.05.033","volume":"122","year":"2005","journal-title":"Cell."},{"key":"2023072907475559100_BIB44","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1111\/j.1365-2958.2004.04388.x","volume":"55","year":"2005","journal-title":"Mol. Microbiol."},{"key":"2023072907475559100_BIB45","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1016\/S1097-2765(03)00181-3","volume":"11","year":"2003","journal-title":"Mol. Cell."},{"key":"2023072907475559100_BIB46","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0092-8674(01)00525-6","volume":"107","year":"2001","journal-title":"Cell."},{"key":"2023072907475559100_BIB47","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1074\/mcp.M300123-MCP200","volume":"3","year":"2004","journal-title":"Mol. Cell. Proteomics."}],"container-title":["The Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/174\/7\/1023\/1875878\/1023.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/174\/7\/1023\/1875878\/1023.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T11:50:43Z","timestamp":1707220243000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/174\/7\/1023\/44582\/Intramembrane-proteolysis-mediates-shedding-of-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,21]]},"references-count":47,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2006,9,25]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.200604136","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1047462.497415","asserted-by":"object"}]},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[2006,9,21]]}}}