{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:30:05Z","timestamp":1791351005586,"version":"4.3.1"},"reference-count":45,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,3,26]]},"abstract":"<jats:p>Spastin, an AAA ATPase mutated in the neurodegenerative disease hereditary spastic paraplegia, severs microtubules. Many other AAA proteins form ring-shaped hexamers and contain pore loops, which project into the ring's central cavity and act as ratchets that pull on target proteins, leading, in some cases, to conformational changes. We show that Spastin assembles into a hexamer and that loops within the central pore recognize C-terminal amino acids of tubulin. Key pore loop amino acids are required for severing, including one altered by a disease-associated mutation. We also show that Spastin contains a second microtubule binding domain that makes a distinct interaction with microtubules and is required for severing. Given that Spastin engages the MT in two places and that both interactions are required for severing, we propose that severing occurs by forces exerted on the C-terminal tail of tubulin, which results in a conformational change in tubulin, which releases it from the polymer.<\/jats:p>","DOI":"10.1083\/jcb.200610072","type":"journal-article","created":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T19:26:22Z","timestamp":1174937182000},"page":"995-1005","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":165,"title":["Recognition of C-terminal amino acids in tubulin by pore loops in Spastin is important for microtubule severing"],"prefix":"10.1083","volume":"176","author":[{"given":"Susan Roehl","family":"White","sequence":"first","affiliation":[{"name":"1Department of Pathology and"},{"name":"2Taub Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia J.","family":"Evans","sequence":"additional","affiliation":[{"name":"1Department of Pathology and"},{"name":"2Taub Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Lary","sequence":"additional","affiliation":[{"name":"3National Analytical Ultracentrifugation Facility and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Cole","sequence":"additional","affiliation":[{"name":"3National Analytical Ultracentrifugation Facility and"},{"name":"4Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Lauring","sequence":"additional","affiliation":[{"name":"1Department of Pathology and"},{"name":"2Taub Institute, College of Physicians and Surgeons, Columbia University, New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2007,3,26]]},"reference":[{"key":"2023072908531554700_BIB1","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.tcb.2005.08.004","volume":"15","year":"2005","journal-title":"Trends Cell Biol."},{"key":"2023072908531554700_BIB2","doi-asserted-by":"crossref","first-page":"2982","DOI":"10.1093\/emboj\/17.11.2982","volume":"17","year":"1998","journal-title":"EMBO J."},{"key":"2023072908531554700_BIB3","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/S0955-0674(01)00289-7","volume":"14","year":"2002","journal-title":"Curr. 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