{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:56:08Z","timestamp":1791269768984,"version":"4.1.0"},"reference-count":51,"publisher":"Rockefeller University Press","issue":"1","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,4,9]]},"abstract":"<jats:p>12R-lipoxygenase (12R-LOX) and the epidermal LOX-3 (eLOX-3) constitute a novel LOX pathway involved in terminal differentiation in skin. This view is supported by recent studies showing that inactivating mutations in 12R-LOX and eLOX-3 are linked to the development of autosomal recessive congenital ichthyosis. We show that 12R-LOX deficiency in mice results in a severe impairment of skin barrier function. Loss of barrier function occurs without alterations in proliferation and stratified organization of the keratinocytes, but is associated with ultrastructural anomalies in the upper granular layer, suggesting perturbance of the assembly\/extrusion of lamellar bodies. Cornified envelopes from skin of 12R-LOX\u2013deficient mice show increased fragility. Lipid analysis demonstrates a disordered composition of ceramides, in particular a decrease of ester-bound ceramide species. Moreover, processing of profilaggrin to monomeric filaggrin is impaired.<\/jats:p>\n                  <jats:p>This study indicates that the 12R-LOX\u2013eLOX-3 pathway plays a key role in the process of epidermal barrier acquisition by affecting lipid metabolism, as well as protein processing.<\/jats:p>","DOI":"10.1083\/jcb.200612116","type":"journal-article","created":{"date-parts":[[2007,4,2]],"date-time":"2007-04-02T20:53:37Z","timestamp":1175547217000},"page":"173-182","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":177,"title":["12R-lipoxygenase deficiency disrupts epidermal barrier function"],"prefix":"10.1083","volume":"177","author":[{"given":"Nikolas","family":"Epp","sequence":"first","affiliation":[{"name":"1Section Eicosanoids and Tumor Development, German Cancer Research Center, D-69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Fu\u0308rstenberger","sequence":"additional","affiliation":[{"name":"1Section Eicosanoids and Tumor Development, German Cancer Research Center, D-69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Mu\u0308ller","sequence":"additional","affiliation":[{"name":"1Section Eicosanoids and Tumor Development, German Cancer Research Center, D-69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"de Juanes","sequence":"additional","affiliation":[{"name":"1Section Eicosanoids and Tumor Development, German Cancer Research Center, D-69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Leitges","sequence":"additional","affiliation":[{"name":"2The Biotechnology Centre of Oslo, University of Oslo, N-0317, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Hausser","sequence":"additional","affiliation":[{"name":"3Dermatological Department, University Clinic Heidelberg, D-69115 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Thieme","sequence":"additional","affiliation":[{"name":"4Institute of Clinical Chemistry, University of Regensburg, D-93042 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Liebisch","sequence":"additional","affiliation":[{"name":"4Institute of Clinical Chemistry, University of Regensburg, D-93042 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Schmitz","sequence":"additional","affiliation":[{"name":"4Institute of Clinical Chemistry, University of Regensburg, D-93042 Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Krieg","sequence":"additional","affiliation":[{"name":"1Section Eicosanoids and Tumor Development, German Cancer Research Center, D-69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2007,4,2]]},"reference":[{"key":"2023072220290331200_BIB1","year":"1992"},{"key":"2023072220290331200_BIB2","doi-asserted-by":"crossref","first-page":"6744","DOI":"10.1073\/pnas.95.12.6744","volume":"95","year":"1998","journal-title":"Proc. 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