{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T20:28:19Z","timestamp":1780086499338,"version":"3.54.0"},"reference-count":44,"publisher":"Rockefeller University Press","issue":"7","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,9,24]]},"abstract":"<jats:p>Bacterial toxins and effector proteins hijack eukaryotic enzymes that are spatially localized and display rapid signaling kinetics. However, the molecular mechanisms by which virulence factors engage highly dynamic substrates in the host cell environment are poorly understood. Here, we demonstrate that the enteropathogenic Escherichia coli (EPEC) type III effector protein EspF nucleates a multiprotein signaling complex composed of eukaryotic sorting nexin 9 (SNX9) and neuronal Wiskott-Aldrich syndrome protein (N-WASP). We demonstrate that a specific and high affinity association between EspF and SNX9 induces membrane remodeling in host cells. These membrane-remodeling events are directly coupled to N-WASP\/Arp2\/3\u2013mediated actin nucleation. In addition to providing a biochemical mechanism of EspF function, we find that EspF dynamically localizes to membrane-trafficking organelles in a spatiotemporal pattern that correlates with SNX9 and N-WASP activity in living cells. Thus, our findings suggest that the EspF-dependent assembly of SNX9 and N-WASP represents a novel form of signaling mimicry used to promote EPEC pathogenesis and gastrointestinal disease.<\/jats:p>","DOI":"10.1083\/jcb.200705021","type":"journal-article","created":{"date-parts":[[2007,9,24]],"date-time":"2007-09-24T18:28:23Z","timestamp":1190658503000},"page":"1265-1278","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":116,"title":["The type III effector EspF coordinates membrane trafficking by the spatiotemporal activation of two eukaryotic signaling pathways"],"prefix":"10.1083","volume":"178","author":[{"given":"Neal M.","family":"Alto","sequence":"first","affiliation":[{"name":"1Departments of Pharmacology, Cellular and Molecular Medicine, and Chemistry and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew W.","family":"Weflen","sequence":"additional","affiliation":[{"name":"3Department of Medicine, Section of Digestive Diseases and Nutrition, University of Illinois at Chicago, Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Rardin","sequence":"additional","affiliation":[{"name":"1Departments of Pharmacology, Cellular and Molecular Medicine, and Chemistry and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Defne","family":"Yarar","sequence":"additional","affiliation":[{"name":"4Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheri S.","family":"Lazar","sequence":"additional","affiliation":[{"name":"1Departments of Pharmacology, Cellular and Molecular Medicine, and Chemistry and Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffi","family":"Tonikian","sequence":"additional","affiliation":[{"name":"5Banting and Best Department of Medical Research and Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 3E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonius","family":"Koller","sequence":"additional","affiliation":[{"name":"2The Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan S.","family":"Taylor","sequence":"additional","affiliation":[{"name":"2The Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Boone","sequence":"additional","affiliation":[{"name":"5Banting and Best Department of Medical Research and Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario M5S 3E1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachdev S.","family":"Sidhu","sequence":"additional","affiliation":[{"name":"6Department of Protein Engineering, Genentech Inc., South San Francisco, CA 94080"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra L.","family":"Schmid","sequence":"additional","affiliation":[{"name":"4Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gail A.","family":"Hecht","sequence":"additional","affiliation":[{"name":"3Department of Medicine, Section of Digestive Diseases and Nutrition, University of Illinois at Chicago, Chicago, IL 60612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack E.","family":"Dixon","sequence":"additional","affiliation":[{"name":"1Departments of Pharmacology, Cellular and Molecular Medicine, and Chemistry and Biochemistry"},{"name":"2The Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2007,9,24]]},"reference":[{"key":"2023072204405033400_BIB1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.cell.2005.10.031","volume":"124","year":"2006","journal-title":"Cell."},{"key":"2023072204405033400_BIB2","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1073\/pnas.0610543104","volume":"104","year":"2007","journal-title":"Proc. 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