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This suggests that another actin nucleation activity is at work in carcinoma cells in response to EGF. A mammalian Diaphanous\u2013related formin, mDia1, localizes at the jagged protrusions in double KD cells. Constitutively active mDia1 recapitulated the phenotype, whereas inhibition of mDia1 blocked the formation of these protrusions. Increased RhoA activity, which stimulates mDia1 nucleation, was observed in the N-WASP\/WAVE2 KD cells and was shown to be required for the N-WASP\/WAVE2 KD phenotype. These data show that coordinate regulation between the WASP family and mDia proteins controls the balance between lamellar and lamellipodial protrusion activity.<\/jats:p>","DOI":"10.1083\/jcb.200708123","type":"journal-article","created":{"date-parts":[[2008,3,24]],"date-time":"2008-03-24T17:34:13Z","timestamp":1206380053000},"page":"1245-1260","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":127,"title":["WASP family members and formin proteins coordinate regulation of cell protrusions in carcinoma cells"],"prefix":"10.1083","volume":"180","author":[{"given":"Corina","family":"Sarmiento","sequence":"first","affiliation":[{"name":"1Department of Anatomy and Structural Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weigang","family":"Wang","sequence":"additional","affiliation":[{"name":"1Department of Anatomy and Structural Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athanassios","family":"Dovas","sequence":"additional","affiliation":[{"name":"1Department of Anatomy and Structural Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"1Department of Anatomy and Structural Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazen","family":"Sidani","sequence":"additional","affiliation":[{"name":"1Department of Anatomy and Structural Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirvat","family":"El-Sibai","sequence":"additional","affiliation":[{"name":"2Department of Pharmacology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"DesMarais","sequence":"additional","affiliation":[{"name":"1Department of Anatomy and Structural Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holly A.","family":"Holman","sequence":"additional","affiliation":[{"name":"4Laboratory of Cell Structure and Signal Integration, Van Andel Research Institute, Grand Rapids, MI 49503"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Kitchen","sequence":"additional","affiliation":[{"name":"4Laboratory of Cell Structure and Signal Integration, Van Andel Research Institute, Grand Rapids, MI 49503"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan M.","family":"Backer","sequence":"additional","affiliation":[{"name":"2Department of Pharmacology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Art","family":"Alberts","sequence":"additional","affiliation":[{"name":"4Laboratory of Cell Structure and Signal Integration, Van Andel Research Institute, Grand Rapids, MI 49503"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Condeelis","sequence":"additional","affiliation":[{"name":"1Department of Anatomy and Structural Biology"},{"name":"3Gruss Lipper Center for Biophotonics, Albert Einstein College of Medicine, Yeshiva University, Bronx, NY 10461"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2008,3,24]]},"reference":[{"key":"2023072220054842800_BIB1","doi-asserted-by":"crossref","first-page":"2824","DOI":"10.1074\/jbc.M006205200","volume":"276","year":"2001","journal-title":"J. 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