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Acute inactivation of chc at stimulated synapses leads to substantial membrane internalization visualized by live dye uptake and electron microscopy. However, chc-inactivated membrane cannot recycle and participate in vesicle release, resulting in a dramatic defect in neurotransmission maintenance during intense synaptic activity. Furthermore, inactivation of chc in the context of other endocytic mutations results in membrane uptake. Our data not only indicate that chc is critical for synaptic vesicle recycling but they also show that in the absence of the protein, bulk retrieval mediates massive synaptic membrane internalization.<\/jats:p>","DOI":"10.1083\/jcb.200804162","type":"journal-article","created":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T20:33:46Z","timestamp":1220301226000},"page":"1007-1016","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":121,"title":["Inactivation of clathrin heavy chain inhibits synaptic recycling but allows bulk membrane uptake"],"prefix":"10.1083","volume":"182","author":[{"given":"Jaroslaw","family":"Kasprowicz","sequence":"first","affiliation":[{"name":"1Department of Molecular and Developmental Genetics, VIB (Flemish Institute for Biotechnology), 3000 Leuven, Belgium"},{"name":"2Laboratory of Neuronal Communication, Program in Cognitive and Molecular Neuroscience, Program in Molecular and Developmental Genetics, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Kuenen","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Developmental Genetics, VIB (Flemish Institute for Biotechnology), 3000 Leuven, Belgium"},{"name":"2Laboratory of Neuronal Communication, Program in Cognitive and Molecular Neuroscience, Program in Molecular and Developmental Genetics, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Miskiewicz","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Developmental Genetics, VIB (Flemish Institute for Biotechnology), 3000 Leuven, Belgium"},{"name":"2Laboratory of Neuronal Communication, Program in Cognitive and Molecular Neuroscience, Program in Molecular and Developmental Genetics, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron L.P.","family":"Habets","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Developmental Genetics, VIB (Flemish Institute for Biotechnology), 3000 Leuven, Belgium"},{"name":"2Laboratory of Neuronal Communication, Program in Cognitive and Molecular Neuroscience, Program in Molecular and Developmental Genetics, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liesbet","family":"Smitz","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Developmental Genetics, VIB (Flemish Institute for Biotechnology), 3000 Leuven, Belgium"},{"name":"2Laboratory of Neuronal Communication, Program in Cognitive and Molecular Neuroscience, Program in Molecular and Developmental Genetics, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrik","family":"Verstreken","sequence":"additional","affiliation":[{"name":"1Department of Molecular and Developmental Genetics, VIB (Flemish Institute for Biotechnology), 3000 Leuven, Belgium"},{"name":"2Laboratory of Neuronal Communication, Program in Cognitive and Molecular Neuroscience, Program in Molecular and Developmental Genetics, Center for Human Genetics, Katholieke Universiteit Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2008,9,1]]},"reference":[{"key":"2023072915044798400_BIB1","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1093\/genetics\/134.4.1119","volume":"134","year":"1993","journal-title":"Genetics."},{"key":"2023072915044798400_BIB2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1126\/science.1553547","volume":"255","year":"1992","journal-title":"Science."},{"key":"2023072915044798400_BIB3","doi-asserted-by":"crossref","first-page":"6964","DOI":"10.1128\/JVI.00024-06","volume":"80","year":"2006","journal-title":"J. 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