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Using the mutational analysis of recombinant subviral particles of tick-borne encephalitis virus, we provide direct experimental evidence that the initiation of fusion is crucially dependent on the protonation of one of the conserved histidines (His323) at the interface between domains I and III of E, leading to the dissolution of domain interactions and to the exposure of the fusion peptide. Conserved histidines located outside this critical interface were found to be completely dispensable for triggering fusion.<\/jats:p>","DOI":"10.1083\/jcb.200806081","type":"journal-article","created":{"date-parts":[[2008,10,20]],"date-time":"2008-10-20T13:53:31Z","timestamp":1224510811000},"page":"353-361","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":173,"title":["Identification of specific histidines as pH sensors in flavivirus membrane fusion"],"prefix":"10.1083","volume":"183","author":[{"given":"Richard","family":"Fritz","sequence":"first","affiliation":[{"name":"Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Stiasny","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franz X.","family":"Heinz","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical University of Vienna, 1095 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2008,10,20]]},"reference":[{"key":"2023072220353061300_BIB1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1007\/BF01703077","volume":"8","year":"1994","journal-title":"Virus Genes."},{"key":"2023072220353061300_BIB2","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1128\/jvi.69.2.695-700.1995","volume":"69","year":"1995","journal-title":"J. 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