{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:04:30Z","timestamp":1791241470517,"version":"4.1.0"},"reference-count":70,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,1,26]]},"abstract":"<jats:p>The actin cytoskeleton is dynamically remodeled during Fc\u03b3 receptor (Fc\u03b3R)-mediated phagocytosis in a phosphatidylinositol (4,5)-bisphosphate (PIP2)-dependent manner. We investigated the role of type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K) \u03b3 and \u03b1 isoforms, which synthesize PIP2, during phagocytosis. PIP5K-\u03b3\u2212\/\u2212 bone marrow\u2013derived macrophages (BMM) have a highly polymerized actin cytoskeleton and are defective in attachment to IgG-opsonized particles and Fc\u03b3R clustering. Delivery of exogenous PIP2 rescued these defects. PIP5K-\u03b3 knockout BMM also have more RhoA and less Rac1 activation, and pharmacological manipulations establish that they contribute to the abnormal phenotype. Likewise, depletion of PIP5K-\u03b3 by RNA interference inhibits particle attachment. In contrast, PIP5K-\u03b1 knockout or silencing has no effect on attachment but inhibits ingestion by decreasing Wiskott-Aldrich syndrome protein activation, and hence actin polymerization, in the nascent phagocytic cup. In addition, PIP5K-\u03b3 but not PIP5K-\u03b1 is transiently activated by spleen tyrosine kinase\u2013mediated phosphorylation. We propose that PIP5K-\u03b3 acts upstream of Rac\/Rho and that the differential regulation of PIP5K-\u03b3 and -\u03b1 allows them to work in tandem to modulate the actin cytoskeleton during the attachment and ingestion phases of phagocytosis.<\/jats:p>","DOI":"10.1083\/jcb.200806121","type":"journal-article","created":{"date-parts":[[2009,1,20]],"date-time":"2009-01-20T02:09:48Z","timestamp":1232417388000},"page":"281-296","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":88,"title":["Essential and unique roles of PIP5K-\u03b3 and -\u03b1 in Fc\u03b3 receptor-mediated phagocytosis"],"prefix":"10.1083","volume":"184","author":[{"given":"Yuntao S.","family":"Mao","sequence":"first","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Yamaga","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Qiao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mia","family":"Yun","sequence":"additional","affiliation":[{"name":"Genentech Inc., South San Francisco, CA 94080 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Pennsylvania, PA 19104 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert","family":"Di Paolo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology, Columbia University, New York, NY 10032 6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bennett","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Mellman","sequence":"additional","affiliation":[{"name":"Genentech Inc., South San Francisco, CA 94080 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles S.","family":"Abrams","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Pennsylvania, PA 19104 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"De Camilli","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Department of Cell Biology, Yale University, New Haven, CT 06520 7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher Y.","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen L.","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Physiology 1 , 2 , and 3"},{"name":"Department of Pathology 1 , 2 , and 3"},{"name":"Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2009,1,19]]},"reference":[{"key":"2023072911440078100_bib1","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1038\/ncb1755","article-title":"Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization","volume":"10","author":"Andrews","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"2023072911440078100_bib2","doi-asserted-by":"crossref","first-page":"4606","DOI":"10.1093\/emboj\/17.16.4606","article-title":"syk protein tyrosine kinase regulates Fc receptor gamma-chain-mediated transport to lysosomes","volume":"17","author":"Bonnerot","year":"1998","journal-title":"EMBO J."},{"key":"2023072911440078100_bib3","doi-asserted-by":"crossref","first-page":"1353","DOI":"10.1083\/jcb.151.7.1353","article-title":"Localized biphasic changes in phosphatidylinositol-4,5-bisphosphate at sites of phagocytosis","volume":"151","author":"Botelho","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib4","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/S0092-8674(04)00003-0","article-title":"Rho and Rac take center stage","volume":"116","author":"Burridge","year":"2004","journal-title":"Cell."},{"key":"2023072911440078100_bib5","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1126\/science.282.5394.1717","article-title":"Identification of two distinct mechanisms of phagocytosis controlled by different Rho GTPases","volume":"282","author":"Caron","year":"1998","journal-title":"Science."},{"key":"2023072911440078100_bib6","doi-asserted-by":"crossref","first-page":"34059","DOI":"10.1074\/jbc.M104917200","article-title":"G-protein-coupled receptor activation induces the membrane translocation and activation of phosphatidylinositol-4-phosphate 5-kinase I alpha by a Rac- and Rho-dependent pathway","volume":"276","author":"Chatah","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib7","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.yexcr.2004.09.005","article-title":"The proto-oncogene Fgr regulates cell migration and this requires its plasma membrane localization","volume":"302","author":"Continolo","year":"2005","journal-title":"Exp. Cell Res."},{"key":"2023072911440078100_bib8","doi-asserted-by":"crossref","first-page":"43849","DOI":"10.1074\/jbc.M209046200","article-title":"Inhibition of phosphatidylinositol-4-phosphate 5-kinase Ialpha impairs localized actin remodeling and suppresses phagocytosis","volume":"277","author":"Coppolino","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib9","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1083\/jcb.200605044","article-title":"Signaling-dependent immobilization of acylated proteins in the inner monolayer of the plasma membrane","volume":"174","author":"Corbett-Nelson","year":"2006","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib11","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1006\/smim.2001.0330","article-title":"Phagocytic signaling strategies: Fc(gamma)receptor-mediated phagocytosis as a model system","volume":"13","author":"Cox","year":"2001","journal-title":"Semin. Immunol."},{"key":"2023072911440078100_bib10","doi-asserted-by":"crossref","first-page":"16597","DOI":"10.1074\/jbc.271.28.16597","article-title":"Syk tyrosine kinase is required for immunoreceptor tyrosine activation motif-dependent actin assembly","volume":"271","author":"Cox","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib12","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1038\/nature05185","article-title":"Phosphoinositides in cell regulation and membrane dynamics","volume":"443","author":"Di Paolo","year":"2006","journal-title":"Nature."},{"key":"2023072911440078100_bib14","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1038\/nature01147","article-title":"Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin","volume":"420","author":"Di Paolo","year":"2002","journal-title":"Nature."},{"key":"2023072911440078100_bib13","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nature02896","article-title":"Impaired PtdIns(4,5)P2 synthesis in nerve terminals produces defects in synaptic vesicle trafficking","volume":"431","author":"Di Paolo","year":"2004","journal-title":"Nature."},{"key":"2023072911440078100_bib15","doi-asserted-by":"crossref","first-page":"28436","DOI":"10.1074\/jbc.274.40.28436","article-title":"Phagosomal maturation, acidification, and inhibition of bacterial growth in nonphagocytic cells transfected with FcgammaRIIA receptors","volume":"274","author":"Downey","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib16","doi-asserted-by":"crossref","first-page":"3048","DOI":"10.4049\/jimmunol.178.5.3048","article-title":"Transmembrane mutations to FcgammaRIIA alter its association with lipid rafts: implications for receptor signaling","volume":"178","author":"Garcia-Garcia","year":"2007","journal-title":"J. Immunol."},{"key":"2023072911440078100_bib17","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1084\/jem.139.2.323","article-title":"Segmental response of the macrophage plasma membrane to a phagocytic stimulus","volume":"139","author":"Griffin","year":"1974","journal-title":"J. Exp. Med."},{"key":"2023072911440078100_bib18","doi-asserted-by":"crossref","first-page":"1957","DOI":"10.1007\/s00018-008-7578-4","article-title":"Molecular mechanisms of phagocytic uptake in mammalian cells","volume":"65","author":"Groves","year":"2008","journal-title":"Cell. Mol. Life Sci."},{"key":"2023072911440078100_bib19","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1038\/ni1337","article-title":"Quantitative proteomic analysis of B cell lipid rafts reveals that ezrin regulates antigen receptor-mediated lipid raft dynamics","volume":"7","author":"Gupta","year":"2006","journal-title":"Nat. Immunol."},{"key":"2023072911440078100_bib20","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1084\/jem.186.6.955","article-title":"Rho is required for the initiation of calcium signaling and phagocytosis by Fcgamma receptors in macrophages","volume":"186","author":"Hackam","year":"1997","journal-title":"J. Exp. Med."},{"key":"2023072911440078100_bib21","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.immuni.2006.02.005","article-title":"Requirements for Vav guanine nucleotide exchange factors and Rho GTPases in FcgammaR- and complement-mediated phagocytosis","volume":"24","author":"Hall","year":"2006","journal-title":"Immunity."},{"key":"2023072911440078100_bib22","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1016\/j.cub.2006.07.066","article-title":"A role for PtdIns(4,5)P2 and PIP5Kalpha in regulating stress-induced apoptosis","volume":"16","author":"Halstead","year":"2006","journal-title":"Curr. Biol."},{"key":"2023072911440078100_bib23","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1091\/mbc.e04-10-0881","article-title":"Actin depolymerization transduces the strength of B-cell receptor stimulation","volume":"16","author":"Hao","year":"2005","journal-title":"Mol. Biol. Cell."},{"key":"2023072911440078100_bib24","doi-asserted-by":"crossref","first-page":"8741","DOI":"10.1074\/jbc.273.15.8741","article-title":"Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion\/substitution analysis of members of this novel lipid kinase family","volume":"273","author":"Ishihara","year":"1998","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib25","doi-asserted-by":"crossref","first-page":"20296","DOI":"10.1073\/pnas.0710258105","article-title":"Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells","volume":"104","author":"Kaizuka","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072911440078100_bib26","doi-asserted-by":"crossref","first-page":"3956","DOI":"10.1128\/MCB.25.10.3956-3966.2005","article-title":"The LIM protein Ajuba regulates phosphatidylinositol 4,5-bisphosphate levels in migrating cells through an interaction with and activation of PIPKI alpha","volume":"25","author":"Kisseleva","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"2023072911440078100_bib27","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1146\/annurev.biophys.34.040204.144637","article-title":"Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules","volume":"34","author":"Kusumi","year":"2005","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"2023072911440078100_bib28","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1242\/jcs.00254","article-title":"Phosphorylation of FcgammaRIIA is required for the receptor-induced actin rearrangement and capping: the role of membrane rafts","volume":"116","author":"Kwiatkowska","year":"2003","journal-title":"J. Cell Sci."},{"key":"2023072911440078100_bib29","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1016\/j.cub.2003.08.005","article-title":"Itk functions to control actin polymerization at the immune synapse through localized activation of Cdc42 and WASP","volume":"13","author":"Labno","year":"2003","journal-title":"Curr. Biol."},{"key":"2023072911440078100_bib30","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1083\/jcb.200705044","article-title":"Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement","volume":"179","author":"Lacalle","year":"2007","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib31","doi-asserted-by":"crossref","first-page":"11135","DOI":"10.1074\/jbc.M700823200","article-title":"Role of CrkII in Fcgamma receptor-mediated phagocytosis","volume":"282","author":"Lee","year":"2007","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib32","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nature01082","article-title":"Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions","volume":"420","author":"Ling","year":"2002","journal-title":"Nature."},{"key":"2023072911440078100_bib33","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1083\/jcb.200310067","article-title":"Tyrosine phosphorylation of type Igamma phosphatidylinositol phosphate kinase by Src regulates an integrin-talin switch","volume":"163","author":"Ling","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib34","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1182\/blood.V95.9.2943.009k17_2943_2946","article-title":"Wiskott-Aldrich syndrome protein is necessary for efficient IgG-mediated phagocytosis","volume":"95","author":"Lorenzi","year":"2000","journal-title":"Blood."},{"key":"2023072911440078100_bib35","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/s00424-007-0286-3","article-title":"Regulation of the actin cytoskeleton by phosphatidylinositol 4-phosphate 5 kinases","volume":"455","author":"Mao","year":"2007","journal-title":"Pflugers Arch."},{"key":"2023072911440078100_bib36","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1038\/35008673","article-title":"Involvement of the Arp2\/3 complex in phagocytosis mediated by FcgammaR or CR3","volume":"2","author":"May","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"2023072911440078100_bib37","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1128\/MCB.24.1.71-83.2004","article-title":"Activation of Syk protein tyrosine kinase in response to osmotic stress requires interaction with p21-activated protein kinase Pak2\/gamma-PAK","volume":"24","author":"Miah","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"2023072911440078100_bib38","doi-asserted-by":"crossref","first-page":"4165","DOI":"10.1182\/blood-2007-08-108886","article-title":"PI5KI-dependent signals are critical regulators of the cytolytic secretory pathway","volume":"111","author":"Micucci","year":"2008","journal-title":"Blood."},{"key":"2023072911440078100_bib39","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1083\/jcb.128.4.589","article-title":"Actin filament disassembly is a sufficient final trigger for exocytosis in nonexcitable cells","volume":"128","author":"Muallem","year":"1995","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib40","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1038\/sj.emboj.7601573","article-title":"Role of activation of PIP5Kgamma661 by AP-2 complex in synaptic vesicle endocytosis","volume":"26","author":"Nakano-Kobayashi","year":"2007","journal-title":"EMBO J."},{"key":"2023072911440078100_bib41","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1038\/nri2206","article-title":"Fcgamma receptors as regulators of immune responses","volume":"8","author":"Nimmerjahn","year":"2008","journal-title":"Nat. Rev. Immunol."},{"key":"2023072911440078100_bib42","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/S0960-9822(02)01069-2","article-title":"Rho-kinase and myosin-II control phagocytic cup formation during CR, but not FcgammaR, phagocytosis","volume":"12","author":"Olazabal","year":"2002","journal-title":"Curr. Biol."},{"key":"2023072911440078100_bib43","doi-asserted-by":"crossref","first-page":"7840","DOI":"10.1074\/jbc.M312737200","article-title":"Activation of type I phosphatidylinositol 4-phosphate 5-kinase isoforms by the Rho GTPases, RhoA, Rac1, and Cdc42","volume":"279","author":"Oude Weernink","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib44","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.ejphar.2004.07.014","article-title":"Regulation and cellular roles of phosphoinositide 5-kinases","volume":"500","author":"Oude Weernink","year":"2004","journal-title":"Eur. J. Pharmacol."},{"key":"2023072911440078100_bib45","doi-asserted-by":"crossref","first-page":"11286","DOI":"10.1073\/pnas.210197897","article-title":"Intracellular delivery of phosphoinositides and inositol phosphates using polyamine carriers","volume":"97","author":"Ozaki","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072911440078100_bib46","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1083\/jcb.200302051","article-title":"Phosphatidylinositol phosphate 5-kinase I\u03b2 recruits AP-2 to the plasma membrane and regulates rates of constitutive endocytosis","volume":"162","author":"Padron","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib47","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1091\/mbc.7.3.435","article-title":"Physical association of the small GTPase Rho with a 68-kDa phosphatidylinositol 4-phosphate 5-kinase in Swiss 3T3 cells","volume":"7","author":"Ren","year":"1996","journal-title":"Mol. Biol. Cell."},{"key":"2023072911440078100_bib48","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/S0092-8674(00)80732-1","article-title":"The interaction between N-WASP and the Arp2\/3 complex links Cdc42-dependent signals to actin assembly","volume":"97","author":"Rohatgi","year":"1999","journal-title":"Cell."},{"key":"2023072911440078100_bib49","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0960-9822(00)00384-5","article-title":"Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2\/3","volume":"10","author":"Rozelle","year":"2000","journal-title":"Curr. Biol."},{"key":"2023072911440078100_bib50","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1084\/jem.20041891","article-title":"Regulation of anaphylactic responses by phosphatidylinositol phosphate kinase type I {alpha}","volume":"201","author":"Sasaki","year":"2005","journal-title":"J. Exp. Med."},{"key":"2023072911440078100_bib51","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1083\/jcb.200412162","article-title":"Phosphatidylinositol-4,5-bisphosphate hydrolysis directs actin remodeling during phagocytosis","volume":"169","author":"Scott","year":"2005","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib52","doi-asserted-by":"crossref","first-page":"4450","DOI":"10.4049\/jimmunol.175.7.4450","article-title":"Binding of IgG-opsonized particles to Fc gamma R is an active stage of phagocytosis that involves receptor clustering and phosphorylation","volume":"175","author":"Sobota","year":"2005","journal-title":"J. Immunol."},{"key":"2023072911440078100_bib53","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/nrm2447","article-title":"Shaping cups into phagosomes and macropinosomes","volume":"9","author":"Swanson","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072911440078100_bib54","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1038\/nrm2069","article-title":"The WASP-WAVE protein network: connecting the membrane to the cytoskeleton","volume":"8","author":"Takenawa","year":"2007","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072911440078100_bib55","doi-asserted-by":"crossref","first-page":"762","DOI":"10.1128\/MCB.18.2.762","article-title":"Characterization of a Rac1- and RhoGDI-associated lipid kinase signaling complex","volume":"18","author":"Tolias","year":"1998","journal-title":"Mol. Cell. Biol."},{"key":"2023072911440078100_bib56","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.jim.2006.10.017","article-title":"Protein transduction as a means of effective manipulation of Cdc42 activity in primary T cells","volume":"319","author":"Tskvitaria-Fuller","year":"2007","journal-title":"J. Immunol. Methods."},{"key":"2023072911440078100_bib57","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1083\/jcb.200107080","article-title":"Vav1\/Rac-dependent actin cytoskeleton reorganization is required for lipid raft clustering in T cells","volume":"155","author":"Villalba","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib59","doi-asserted-by":"crossref","first-page":"11748","DOI":"10.1073\/pnas.0700019104","article-title":"PIP5KI gamma is required for cardiovascular and neuronal development","volume":"104","author":"Wang","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072911440078100_bib58","doi-asserted-by":"crossref","first-page":"14064","DOI":"10.1073\/pnas.0804139105","article-title":"Loss of PIP5KIbeta demonstrates that PIP5KI isoform-specific PIP2 synthesis is required for IP3 formation","volume":"105","author":"Wang","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072911440078100_bib60","first-page":"812","article-title":"Loss of PIP5KIgamma, unlike other PIP5KI isoforms, impairs the integrity of the membrane cytoskeleton in murine megakaryocytes","volume":"118","author":"Wang","year":"2008","journal-title":"J. Clin. Invest."},{"key":"2023072911440078100_bib62","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S0092-8674(03)00603-2","article-title":"Phosphatidylinositol 4 phosphate regulates targeting of clathrin adaptor AP-1 complexes to the Golgi","volume":"114","author":"Wang","year":"2003","journal-title":"Cell."},{"key":"2023072911440078100_bib61","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1083\/jcb.200408008","article-title":"Critical role of PIP5KI\u03b387 in InsP3-mediated Ca(2+) signaling","volume":"167","author":"Wang","year":"2004","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib63","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.1242\/jcs.00997","article-title":"Rac1-deficient macrophages exhibit defects in cell spreading and membrane ruffling but not migration","volume":"117","author":"Wells","year":"2004","journal-title":"J. Cell Sci."},{"key":"2023072911440078100_bib64","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/S0896-6273(01)00456-1","article-title":"PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse","volume":"32","author":"Wenk","year":"2001","journal-title":"Neuron."},{"key":"2023072911440078100_bib65","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1242\/jcs.03024","article-title":"Rac1 and Rac2 regulate macrophage morphology but are not essential for migration","volume":"119","author":"Wheeler","year":"2006","journal-title":"J. Cell Sci."},{"key":"2023072911440078100_bib67","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1083\/jcb.152.5.867","article-title":"Phosphatidylinositol 4,5-bisphosphate induces actin stress-fiber formation and inhibits membrane ruffling in CV1 cells","volume":"152","author":"Yamamoto","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072911440078100_bib66","doi-asserted-by":"crossref","first-page":"32630","DOI":"10.1074\/jbc.M605928200","article-title":"Hypertonic stress increases phosphatidylinositol 4,5-bisphosphate levels by activating PIP5KIbeta","volume":"281","author":"Yamamoto","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib68","doi-asserted-by":"crossref","first-page":"42331","DOI":"10.1074\/jbc.M404335200","article-title":"Rho and Rho-kinase mediate thrombin-induced phosphatidylinositol 4-phosphate 5-kinase trafficking in platelets","volume":"279","author":"Yang","year":"2004","journal-title":"J. Biol. Chem."},{"key":"2023072911440078100_bib69","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/j.ceb.2006.06.006","article-title":"Lipid metabolism and dynamics during phagocytosis","volume":"18","author":"Yeung","year":"2006","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072911440078100_bib70","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1146\/annurev.physiol.65.092101.142517","article-title":"Phosphoinositide regulation of the actin cytoskeleton","volume":"65","author":"Yin","year":"2003","journal-title":"Annu. Rev. Physiol."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/184\/2\/281\/1889447\/jcb_200806121.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/184\/2\/281\/1889447\/jcb_200806121.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T11:46:31Z","timestamp":1690631191000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/184\/2\/281\/54040\/Essential-and-unique-roles-of-PIP5K-and-in-Fc"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,19]]},"references-count":70,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,1,26]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.200806121","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[2009,1,19]]}}}