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We show that activation of the G protein\u2013coupled receptor (GPCR) trapped in endoderm 1 (Tre1) directs the redistribution of the G protein G\u03b2 as well as adherens junction proteins and Rho guanosine triphosphatase from the cell periphery to the lagging tail of germ cells at the onset of Drosophila melanogaster germ cell migration. Subsequently, Tre1 activity triggers germ cell dispersal and orients them toward the midgut for directed transepithelial migration. A transition toward invasive migration is also a prerequisite for metastasis formation, which often correlates with down-regulation of adhesion proteins. We show that uniform down-regulation of E-cadherin causes germ cell dispersal but is not sufficient for transepithelial migration in the absence of Tre1. Our findings therefore suggest a new mechanism for GPCR function that links cell polarity, modulation of cell adhesion, and invasion.<\/jats:p>","DOI":"10.1083\/jcb.200807049","type":"journal-article","created":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T17:06:48Z","timestamp":1222880808000},"page":"157-168","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":83,"title":["Tre1 GPCR initiates germ cell transepithelial migration by regulating <i>Drosophila melanogaster<\/i> E-cadherin"],"prefix":"10.1083","volume":"183","author":[{"given":"Prabhat S.","family":"Kunwar","sequence":"first","affiliation":[{"name":"1Howard Hughes Medical Institute"},{"name":"2Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Sano","sequence":"additional","affiliation":[{"name":"1Howard Hughes Medical Institute"},{"name":"2Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Renault","sequence":"additional","affiliation":[{"name":"1Howard Hughes Medical Institute"},{"name":"2Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor","family":"Barbosa","sequence":"additional","affiliation":[{"name":"1Howard Hughes Medical Institute"},{"name":"2Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoyuki","family":"Fuse","sequence":"additional","affiliation":[{"name":"3Global Center of Excellence Program, Division of Biological Science, Kyoto University, Kyoto 606-8502, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Lehmann","sequence":"additional","affiliation":[{"name":"1Howard Hughes Medical Institute"},{"name":"2Kimmel Center for Biology and Medicine at the Skirball Institute for Biomolecular Medicine, Department of Cell Biology, New York University School of Medicine, New York, NY 10016"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2008,9,29]]},"reference":[{"key":"2023072915092869100_BIB1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.devcel.2005.06.003","volume":"9","year":"2005","journal-title":"Dev. 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