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At these sites, the ER protein VAP (VAMP [vesicle-associated membrane protein]-associated ER protein) can interact in trans with the Rab7\u2013RILP complex to remove p150Glued and associated motors. LEs then move to the microtubule plus end. Under high cholesterol conditions, as in Niemann-Pick type C disease, this process is prevented, and LEs accumulate at the microtubule minus end as the result of dynein motor activity. These data explain how the ER and cholesterol control the association of LEs with motor proteins and their positioning in cells.<\/jats:p>","DOI":"10.1083\/jcb.200811005","type":"journal-article","created":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T12:29:43Z","timestamp":1246278583000},"page":"1209-1225","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":685,"title":["Cholesterol sensor ORP1L contacts the ER protein VAP to control Rab7\u2013RILP\u2013p150Glued and late endosome positioning"],"prefix":"10.1083","volume":"185","author":[{"given":"Nuno","family":"Rocha","sequence":"first","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coenraad","family":"Kuijl","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rik","family":"van der Kant","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennert","family":"Janssen","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Houben","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans","family":"Janssen","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilbert","family":"Zwart","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Neefjes","sequence":"additional","affiliation":[{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"},{"name":"Division of Cell Biology 1 and 2"},{"name":"Centre for Biomedical Genetics, The Netherlands Cancer Institute, 1066CX Amsterdam, Netherlands 1 and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2009,6,29]]},"reference":[{"key":"2023072912311220000_bib1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/S0962-8924(98)01410-X","article-title":"Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell","volume":"9","author":"Bastiaens","year":"1999","journal-title":"Trends Cell Biol."},{"key":"2023072912311220000_bib2","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1111\/j.1600-0854.2005.00347.x","article-title":"Kinesin-2 is a motor for late endosomes and lysosomes","volume":"6","author":"Brown","year":"2005","journal-title":"Traffic."},{"key":"2023072912311220000_bib4","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1083\/jcb.139.2.469","article-title":"Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution","volume":"139","author":"Burkhardt","year":"1997","journal-title":"J. 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