{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:55:56Z","timestamp":1779353756271,"version":"3.51.4"},"reference-count":73,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,11,30]]},"abstract":"<jats:p>Indian hedgehog (Ihh) regulates proliferation and differentiation of chondrocytes in the growth plate. Although the biology of Ihh is currently well documented, its transcriptional regulation is poorly understood. \u03b4-EF1 is a two-handed zinc finger\/homeodomain transcriptional repressor. Targeted inactivation of mouse \u03b4-EF1 leads to skeletal abnormalities including disorganized growth plates, shortening of long bones, and joint fusions, which are reminiscent of defects associated with deregulation of Ihh signaling. Here, we show that the absence of \u03b4-EF1 results in delayed hypertrophic differentiation of chondrocytes and increased cell proliferation in the growth plate. Further, we demonstrate that \u03b4-EF1 binds to the putative regulatory elements in intron 1 of Ihh in vitro and in vivo, resulting in down-regulation of Ihh expression. Finally, we show that \u03b4-EF1 haploinsufficiency leads to a postnatal increase in trabecular bone mass associated with enhanced Ihh expression. In summary, we have identified \u03b4-EF1 as an in vivo negative regulator of Ihh expression in the growth plate.<\/jats:p>","DOI":"10.1083\/jcb.200904034","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T02:56:34Z","timestamp":1259031394000},"page":"685-699","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["\u03b4-EF1 is a negative regulator of <i>Ihh<\/i> in the developing growth plate"],"prefix":"10.1083","volume":"187","author":[{"given":"Ellen","family":"Bellon","sequence":"first","affiliation":[{"name":"Laboratory of Skeletal Development and Joint Disorders, Division of Rheumatology, Department of Musculoskeletal Sciences, University of Leuven, Leuven 3000, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank P.","family":"Luyten","sequence":"additional","affiliation":[{"name":"Laboratory of Skeletal Development and Joint Disorders, Division of Rheumatology, Department of Musculoskeletal Sciences, University of Leuven, Leuven 3000, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Przemko","family":"Tylzanowski","sequence":"additional","affiliation":[{"name":"Laboratory of Skeletal Development and Joint Disorders, Division of Rheumatology, Department of Musculoskeletal Sciences, University of Leuven, Leuven 3000, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2009,11,23]]},"reference":[{"key":"2023072912510532000_bib1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1042\/bj3360327","article-title":"Functional characterization of transcriptional regulatory elements in the upstream region and intron 1 of the human S6 ribosomal protein gene","volume":"336","author":"Antoine","year":"1998","journal-title":"Biochem. J."},{"key":"2023072912510532000_bib2","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1038\/8792","article-title":"Sox9 is required for cartilage formation","volume":"22","author":"Bi","year":"1999","journal-title":"Nat. Genet."},{"key":"2023072912510532000_bib3","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1006\/dbio.1995.0010","article-title":"Hedgehog and Bmp genes are coexpressed at many diverse sites of cell-cell interaction in the mouse embryo","volume":"172","author":"Bitgood","year":"1995","journal-title":"Dev. Biol."},{"key":"2023072912510532000_bib5","doi-asserted-by":"publisher","first-page":"643","DOI":"10.1089\/dna.1996.15.643","article-title":"Alternative splicing gives rise to two isoforms of Zfhep, a zinc finger\/homeodomain protein that binds T3-response elements","volume":"15","author":"Cabanillas","year":"1996","journal-title":"DNA Cell Biol."},{"key":"2023072912510532000_bib6","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/S0092-8674(00)81374-4","article-title":"Dual roles for patched in sequestering and transducing Hedgehog","volume":"87","author":"Chen","year":"1996","journal-title":"Cell."},{"key":"2023072912510532000_bib7","doi-asserted-by":"publisher","first-page":"3788","DOI":"10.1002\/art.23985","article-title":"Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone-related protein in mice","volume":"58","author":"Chen","year":"2008","journal-title":"Arthritis Rheum."},{"key":"2023072912510532000_bib9","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1002\/ajmg.a.20495","article-title":"The hedgehog signaling network","volume":"123A","author":"Cohen","year":"2003","journal-title":"Am. J. Med. Genet. A."},{"key":"2023072912510532000_bib10","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1242\/dev.01649","article-title":"Indian hedgehog synchronizes skeletal angiogenesis and perichondrial maturation with cartilage development","volume":"132","author":"Colnot","year":"2005","journal-title":"Development."},{"key":"2023072912510532000_bib11","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1016\/S1097-2765(01)00260-X","article-title":"The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion","volume":"7","author":"Comijn","year":"2001","journal-title":"Mol. Cell."},{"key":"2023072912510532000_bib12","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1177\/002215540205000808","article-title":"Distribution of the transcription factors Sox9, AP-2, and [delta]EF1 in adult murine articular and meniscal cartilage and growth plate","volume":"50","author":"Davies","year":"2002","journal-title":"J. Histochem. Cytochem."},{"key":"2023072912510532000_bib13","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1089\/104454904322745907","article-title":"Transcriptional activation by the zinc-finger homeodomain protein delta EF1 in estrogen signaling cascades","volume":"23","author":"Dillner","year":"2004","journal-title":"DNA Cell Biol."},{"key":"2023072912510532000_bib14","doi-asserted-by":"publisher","first-page":"1304","DOI":"10.1101\/gr.142200","article-title":"Active conservation of noncoding sequences revealed by three-way species comparisons","volume":"10","author":"Dubchak","year":"2000","journal-title":"Genome Res."},{"key":"2023072912510532000_bib16","doi-asserted-by":"publisher","first-page":"2375","DOI":"10.1038\/sj.onc.1208429","article-title":"DeltaEF1 is a transcriptional repressor of E-cadherin and regulates epithelial plasticity in breast cancer cells","volume":"24","author":"Eger","year":"2005","journal-title":"Oncogene."},{"key":"2023072912510532000_bib17","first-page":"6773","article-title":"BZP, a novel serum-responsive zinc finger protein that inhibits gene transcription","volume":"14","author":"Franklin","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"2023072912510532000_bib18","doi-asserted-by":"publisher","first-page":"W273","DOI":"10.1093\/nar\/gkh458","article-title":"VISTA: computational tools for comparative genomics","volume":"32","author":"Frazer","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"2023072912510532000_bib19","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1242\/dev.119.2.433","article-title":"Delta-crystallin enhancer binding protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis","volume":"119","author":"Funahashi","year":"1993","journal-title":"Development."},{"key":"2023072912510532000_bib20","first-page":"6153","article-title":"Displacement of an E-box-binding repressor by basic helix-loop-helix proteins: implications for B-cell specificity of the immunoglobulin heavy-chain enhancer","volume":"14","author":"Genetta","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"2023072912510532000_bib21","doi-asserted-by":"publisher","first-page":"e6067","DOI":"10.1371\/journal.pone.0006067","article-title":"The Wnt\/beta-catenin pathway interacts differentially with PTHrP signaling to control chondrocyte hypertrophy and final maturation","volume":"4","author":"Guo","year":"2009","journal-title":"PLoS One."},{"key":"2023072912510532000_bib22","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1002\/aja.1001950404","article-title":"A series of normal stages in the development of the chick embryo. 1951","volume":"195","author":"Hamburger","year":"1992","journal-title":"Dev. Dyn."},{"key":"2023072912510532000_bib23","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/S0168-9525(96)10051-2","article-title":"The world according to hedgehog","volume":"13","author":"Hammerschmidt","year":"1997","journal-title":"Trends Genet."},{"key":"2023072912510532000_bib24","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.ydbio.2007.05.011","article-title":"Tamoxifen-inducible gene deletion reveals a distinct cell type associated with trabecular bone, and direct regulation of PTHrP expression and chondrocyte morphology by Ihh in growth region cartilage","volume":"308","author":"Hilton","year":"2007","journal-title":"Dev. Biol."},{"key":"2023072912510532000_bib25","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1006\/viro.1998.9290","article-title":"The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses","volume":"248","author":"Himly","year":"1998","journal-title":"Virology."},{"key":"2023072912510532000_bib26","article-title":"Manipulating the mouse embryo","author":"Hogan","year":"2004"},{"key":"2023072912510532000_bib27","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/S0925-4773(97)00145-7","article-title":"Expression of indian hedgehog, bone morphogenetic protein 6 and gli during skeletal morphogenesis","volume":"69","author":"Iwasaki","year":"1997","journal-title":"Mech. Dev."},{"key":"2023072912510532000_bib28","doi-asserted-by":"publisher","first-page":"36037","DOI":"10.1074\/jbc.M508698200","article-title":"Alpha7 integrin expression is negatively regulated by deltaEF1 during skeletal myogenesis","volume":"280","author":"Jethanandani","year":"2005","journal-title":"J. Biol. Chem."},{"key":"2023072912510532000_bib29","first-page":"5206","article-title":"Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer","volume":"13","author":"Kamachi","year":"1993","journal-title":"Mol. Cell. Biol."},{"key":"2023072912510532000_bib30","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1242\/dev.127.3.543","article-title":"Indian hedgehog coordinates endochondral bone growth and morphogenesis via parathyroid hormone related-protein-dependent and -independent pathways","volume":"127","author":"Karp","year":"2000","journal-title":"Development."},{"key":"2023072912510532000_bib31","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1677\/joe.0.174R001","article-title":"Expression and localization of Indian hedgehog (Ihh) and parathyroid hormone related protein (PTHrP) in the human growth plate during pubertal development","volume":"174","author":"Kindblom","year":"2002","journal-title":"J. Endocrinol."},{"key":"2023072912510532000_bib32","doi-asserted-by":"publisher","first-page":"1734","DOI":"10.1172\/JCI24397","article-title":"Indian hedgehog stimulates periarticular chondrocyte differentiation to regulate growth plate length independently of PTHrP","volume":"115","author":"Kobayashi","year":"2005","journal-title":"J. Clin. Invest."},{"key":"2023072912510532000_bib33","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/j.devcel.2004.05.009","article-title":"Ext1-dependent heparan sulfate regulates the range of Ihh signaling during endochondral ossification","volume":"6","author":"Koziel","year":"2004","journal-title":"Dev. Cell."},{"key":"2023072912510532000_bib34","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1038\/nature01657","article-title":"Developmental regulation of the growth plate","volume":"423","author":"Kronenberg","year":"2003","journal-title":"Nature."},{"key":"2023072912510532000_bib35","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1172\/JCI6629","article-title":"Ablation of the PTHrP gene or the PTH\/PTHrP receptor gene leads to distinct abnormalities in bone development","volume":"104","author":"Lanske","year":"1999","journal-title":"J. Clin. Invest."},{"key":"2023072912510532000_bib36","doi-asserted-by":"publisher","first-page":"2152","DOI":"10.1002\/ajmg.a.32441","article-title":"Deletion of 1 amino acid in Indian hedgehog leads to brachydactylyA1","volume":"146A","author":"Lodder","year":"2008","journal-title":"Am. J. Med. Genet. A."},{"key":"2023072912510532000_bib37","doi-asserted-by":"crossref","first-page":"5099","DOI":"10.1242\/dev.128.24.5099","article-title":"Genetic manipulation of hedgehog signaling in the endochondral skeleton reveals a direct role in the regulation of chondrocyte proliferation","volume":"128","author":"Long","year":"2001","journal-title":"Development."},{"key":"2023072912510532000_bib38","doi-asserted-by":"publisher","first-page":"1309","DOI":"10.1242\/dev.01006","article-title":"Ihh signaling is directly required for the osteoblast lineage in the endochondral skeleton","volume":"131","author":"Long","year":"2004","journal-title":"Development."},{"key":"2023072912510532000_bib39","doi-asserted-by":"publisher","first-page":"6382","DOI":"10.1073\/pnas.0608449104","article-title":"Indian Hedgehog produced by postnatal chondrocytes is essential for maintaining a growth plate and trabecular bone","volume":"104","author":"Maeda","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072912510532000_bib40","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1016\/j.devcel.2008.02.003","article-title":"Hedgehog signaling in mature osteoblasts regulates bone formation and resorption by controlling PTHrP and RANKL expression","volume":"14","author":"Mak","year":"2008","journal-title":"Dev. Cell."},{"key":"2023072912510532000_bib41","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1038\/384176a0","article-title":"Biochemical evidence that patched is the Hedgehog receptor","volume":"384","author":"Marigo","year":"1996","journal-title":"Nature."},{"key":"2023072912510532000_bib42","doi-asserted-by":"publisher","first-page":"7852","DOI":"10.1038\/sj.onc.1203243","article-title":"Gli genes in development and cancer","volume":"18","author":"Matise","year":"1999","journal-title":"Oncogene."},{"key":"2023072912510532000_bib43","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1016\/S0962-8924(02)02405-4","article-title":"Pathways and consequences: Hedgehog signaling in human disease","volume":"12","author":"Mullor","year":"2002","journal-title":"Trends Cell Biol."},{"key":"2023072912510532000_bib44","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1007\/PL00005843","article-title":"Expression of Indian hedgehog during fracture healing in adult rat femora","volume":"66","author":"Murakami","year":"2000","journal-title":"Calcif. Tissue Int."},{"key":"2023072912510532000_bib45","doi-asserted-by":"publisher","first-page":"3610","DOI":"10.1074\/jbc.275.5.3610","article-title":"The transcription factor deltaEF1 is inversely expressed with type II collagen mRNA and can repress Col2a1 promoter activity in transfected chondrocytes","volume":"275","author":"Murray","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2023072912510532000_bib46","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1111\/j.1399-0004.2004.00360.x","article-title":"Hedgehog signaling and congenital malformations","volume":"67","author":"Nieuwenhuis","year":"2005","journal-title":"Clin. Genet."},{"key":"2023072912510532000_bib47","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.devcel.2006.05.011","article-title":"DeltaEF1 mediates TGF-beta signaling in vascular smooth muscle cell differentiation","volume":"11","author":"Nishimura","year":"2006","journal-title":"Dev. Cell."},{"key":"2023072912510532000_bib48","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1016\/j.devcel.2008.03.007","article-title":"Patched1 haploinsufficiency increases adult bone mass and modulates Gli3 repressor activity","volume":"14","author":"Ohba","year":"2008","journal-title":"Dev. Cell."},{"key":"2023072912510532000_bib49","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/S0925-4773(01)00599-8","article-title":"Dynamic regulation of Brachyury expression in the amphibian embryo by XSIP1","volume":"111","author":"Papin","year":"2002","journal-title":"Mech. Dev."},{"key":"2023072912510532000_bib51","doi-asserted-by":"publisher","first-page":"3935","DOI":"10.1093\/emboj\/16.13.3935","article-title":"ZEB, a vertebrate homolog of Drosophila Zfh-1, is a negative regulator of muscle differentiation","volume":"16","author":"Postigo","year":"1997","journal-title":"EMBO J."},{"key":"2023072912510532000_bib52","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1016\/j.bbrc.2004.11.068","article-title":"Molecular mechanisms of endochondral bone development","volume":"328","author":"Provot","year":"2005","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2023072912510532000_bib53","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1242\/dev.02258","article-title":"Nkx3.2\/Bapx1 acts as a negative regulator of chondrocyte maturation","volume":"133","author":"Provot","year":"2006","journal-title":"Development."},{"key":"2023072912510532000_bib54","doi-asserted-by":"publisher","first-page":"4299","DOI":"10.1073\/pnas.87.11.4299","article-title":"Regulatory elements in the introns of the human HPRT gene are necessary for its expression in embryonic stem cells","volume":"87","author":"Reid","year":"1990","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072912510532000_bib55","doi-asserted-by":"publisher","first-page":"5073","DOI":"10.1093\/emboj\/18.18.5073","article-title":"New mode of DNA binding of multi-zinc finger transcription factors: deltaEF1 family members bind with two hands to two target sites","volume":"18","author":"Remacle","year":"1999","journal-title":"EMBO J."},{"key":"2023072912510532000_bib56","doi-asserted-by":"publisher","first-page":"3231","DOI":"10.1242\/dev.02480","article-title":"Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors","volume":"133","author":"Rodda","year":"2006","journal-title":"Development."},{"key":"2023072912510532000_bib57","first-page":"305","article-title":"Development of immortalized cells derived from 13DPC mouse limb buds as a system to study the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) on limb bud cell differentiation","volume":"383A","author":"Rosen","year":"1993","journal-title":"Prog. Clin. Biol. Res."},{"key":"2023072912510532000_bib1994","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1101\/gad.8.11.1311","article-title":"Xenopus embryos regulate the nuclear localization of XMyoD","volume":"8","author":"Rupp","year":"1994","journal-title":"Genes Dev."},{"key":"2023072912510532000_bib58","first-page":"5692","article-title":"The delta-crystallin enhancer-binding protein delta EF1 is a repressor of E2-box-mediated gene activation","volume":"14","author":"Sekido","year":"1994","journal-title":"Mol. Cell. Biol."},{"key":"2023072912510532000_bib59","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/0378-1119(96)00185-0","article-title":"Organization of the gene encoding transcriptional repressor deltaEF1 and cross-species conservation of its domains","volume":"173","author":"Sekido","year":"1996","journal-title":"Gene."},{"key":"2023072912510532000_bib60","doi-asserted-by":"publisher","first-page":"771","DOI":"10.1046\/j.1365-2443.1997.1570355.x","article-title":"Two mechanisms in the action of repressor deltaEF1: binding site competition with an activator and active repression","volume":"2","author":"Sekido","year":"1997","journal-title":"Genes Cells."},{"key":"2023072912510532000_bib61","doi-asserted-by":"publisher","first-page":"9947","DOI":"10.1074\/jbc.271.17.9947","article-title":"A regulatory element in intron 1 of the cystic fibrosis transmembrane conductance regulator gene","volume":"271","author":"Smith","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2023072912510532000_bib62","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1083\/jcb.200312045","article-title":"Sox5 and Sox6 are needed to develop and maintain source, columnar, and hypertrophic chondrocytes in the cartilage growth plate","volume":"164","author":"Smits","year":"2004","journal-title":"J. Cell Biol."},{"key":"2023072912510532000_bib63","doi-asserted-by":"publisher","first-page":"3039","DOI":"10.1242\/dev.02471","article-title":"Wnt9a signaling is required for joint integrity and regulation of Ihh during chondrogenesis","volume":"133","author":"Sp\u00e4ter","year":"2006","journal-title":"Development."},{"key":"2023072912510532000_bib64","doi-asserted-by":"publisher","first-page":"2072","DOI":"10.1101\/gad.13.16.2072","article-title":"Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation","volume":"13","author":"St-Jacques","year":"1999","journal-title":"Genes Dev."},{"key":"2023072912510532000_bib65","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1038\/384129a0","article-title":"The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog","volume":"384","author":"Stone","year":"1996","journal-title":"Nature."},{"key":"2023072912510532000_bib66","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1242\/dev.125.1.21","article-title":"DeltaEF1, a zinc finger and homeodomain transcription factor, is required for skeleton patterning in multiple lineages","volume":"125","author":"Takagi","year":"1998","journal-title":"Development."},{"key":"2023072912510532000_bib67","doi-asserted-by":"publisher","first-page":"1678","DOI":"10.1359\/JBMR.040706","article-title":"Targeted expression of SHH affects chondrocyte differentiation, growth plate organization, and Sox9 expression","volume":"19","author":"Tavella","year":"2004","journal-title":"J. Bone Miner. Res."},{"key":"2023072912510532000_bib68","doi-asserted-by":"publisher","first-page":"37011","DOI":"10.1074\/jbc.M104185200","article-title":"delta Ef1 binds to a far upstream sequence of the mouse pro-alpha 1(I) collagen gene and represses its expression in osteoblasts","volume":"276","author":"Terraz","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072912510532000_bib69","doi-asserted-by":"publisher","first-page":"1019","DOI":"10.1016\/j.mod.2004.05.015","article-title":"The contribution of chicken embryology to the understanding of vertebrate limb development","volume":"121","author":"Tickle","year":"2004","journal-title":"Mech. Dev."},{"key":"2023072912510532000_bib70","doi-asserted-by":"publisher","first-page":"40001","DOI":"10.1074\/jbc.M104112200","article-title":"Smad-interacting protein 1 is a repressor of liver\/bone\/kidney alkaline phosphatase transcription in bone morphogenetic protein-induced osteogenic differentiation of C2C12 cells","volume":"276","author":"Tylzanowski","year":"2001","journal-title":"J. Biol. Chem."},{"key":"2023072912510532000_bib71","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/S1567-133X(02)00092-3","article-title":"Zfhx1a and Zfhx1b mRNAs have non-overlapping expression domains during chick and mouse midgestation limb development","volume":"3","author":"Tylzanowski","year":"2003","journal-title":"Gene Expr. Patterns."},{"key":"2023072912510532000_bib72","doi-asserted-by":"publisher","first-page":"20489","DOI":"10.1074\/jbc.274.29.20489","article-title":"SIP1, a novel zinc finger\/homeodomain repressor, interacts with Smad proteins and binds to 5\u2032-CACCT sequences in candidate target genes","volume":"274","author":"Verschueren","year":"1999","journal-title":"J. Biol. Chem."},{"key":"2023072912510532000_bib73","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1126\/science.273.5275.613","article-title":"Regulation of rate of cartilage differentiation by Indian hedgehog and PTH-related protein","volume":"273","author":"Vortkamp","year":"1996","journal-title":"Science."},{"key":"2023072912510532000_bib74","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1093\/oxfordjournals.jbchem.a124267","article-title":"Transcription factors positively and negatively regulating the Na,K-ATPase alpha 1 subunit gene","volume":"114","author":"Watanabe","year":"1993","journal-title":"J. Biochem."},{"key":"2023072912510532000_bib75","doi-asserted-by":"publisher","first-page":"1791","DOI":"10.1126\/science.1840704","article-title":"Identification of a zinc finger protein that inhibits IL-2 gene expression","volume":"254","author":"Williams","year":"1991","journal-title":"Science."},{"key":"2023072912510532000_bib76","doi-asserted-by":"publisher","first-page":"952","DOI":"10.1101\/gad.1174704","article-title":"Runx2 and Runx3 are essential for chondrocyte maturation, and Runx2 regulates limb growth through induction of Indian hedgehog","volume":"18","author":"Yoshida","year":"2004","journal-title":"Genes Dev."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/187\/5\/685\/1893364\/jcb_200904034.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/187\/5\/685\/1893364\/jcb_200904034.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T12:52:20Z","timestamp":1690635140000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/187\/5\/685\/35707\/EF1-is-a-negative-regulator-of-Ihh-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,23]]},"references-count":73,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,11,30]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.200904034","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[2009,11,23]]}}}