{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:57:51Z","timestamp":1791233871843,"version":"4.1.0"},"reference-count":63,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,2,8]]},"abstract":"<jats:p>In Caenorhabditis elegans, five proteins are required for centriole duplication: SPD-2, ZYG-1, SAS-5, SAS-6, and SAS-4. Functional orthologues of all but SAS-5 have been found in other species. In Drosophila melanogaster and humans, Sak\/Plk4, DSas-6\/hSas-6, and DSas-4\/CPAP\u2014orthologues of ZYG-1, SAS-6, and SAS-4, respectively\u2014are required for centriole duplication. Strikingly, all three fly proteins can induce the de novo formation of centriole-like structures when overexpressed in unfertilized eggs. Here, we find that of eight candidate duplication factors identified in cultured fly cells, only two, Ana2 and Asterless (Asl), share this ability. Asl is now known to be essential for centriole duplication in flies, but no equivalent protein has been found in worms. We show that Ana2 is the likely functional orthologue of SAS-5 and that it is also related to the vertebrate STIL\/SIL protein family that has been linked to microcephaly in humans. We propose that members of the SAS-5\/Ana2\/STIL family of proteins are key conserved components of the centriole duplication machinery.<\/jats:p>","DOI":"10.1083\/jcb.200910016","type":"journal-article","created":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T21:49:29Z","timestamp":1265060969000},"page":"313-323","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":139,"title":["Drosophila Ana2 is a conserved centriole duplication factor"],"prefix":"10.1083","volume":"188","author":[{"given":"Naomi R.","family":"Stevens","sequence":"first","affiliation":[{"name":"The Gurdon Institute, Cambridge, England CB2 1QN, UK"},{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, England OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen","family":"Dobbelaere","sequence":"additional","affiliation":[{"name":"The Gurdon Institute, Cambridge, England CB2 1QN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrin","family":"Brunk","sequence":"additional","affiliation":[{"name":"The Gurdon Institute, Cambridge, England CB2 1QN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Franz","sequence":"additional","affiliation":[{"name":"The Gurdon Institute, Cambridge, England CB2 1QN, UK"},{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, England OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan W.","family":"Raff","sequence":"additional","affiliation":[{"name":"The Gurdon Institute, Cambridge, England CB2 1QN, UK"},{"name":"Sir William Dunn School of Pathology, University of Oxford, Oxford, England OX1 3RE, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2010,2,1]]},"reference":[{"key":"2020100518491429500_bib1","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"2020100518491429500_bib2","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1038\/nrg1557","article-title":"The centrosome in human genetic disease","volume":"6","author":"Badano","year":"2005","journal-title":"Nat. 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