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Although structurally intact, centrosomes lacking the CNN1 domain fail to recruit specific PCM components that mediate attachment to spindle poles. Furthermore, we show that the CNN1 domain enforces cohesion between parental centrioles during interphase and promotes efficient DNA damage\u2013induced G2 cell cycle arrest. Because mitotic spindle positioning, asymmetric centrosome inheritance, and DNA damage signaling have all been implicated in cell fate determination during neurogenesis, our findings provide novel insight into how impaired CDK5RAP2 function could cause premature depletion of neural stem cells and thereby microcephaly.<\/jats:p>","DOI":"10.1083\/jcb.200912163","type":"journal-article","created":{"date-parts":[[2010,4,5]],"date-time":"2010-04-05T20:44:18Z","timestamp":1270500258000},"page":"23-39","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":116,"title":["CDK5RAP2 functions in centrosome to spindle pole attachment and DNA damage response"],"prefix":"10.1083","volume":"189","author":[{"given":"Alexis R.","family":"Barr","sequence":"first","affiliation":[{"name":"Cancer Research UK Cambridge Research Institute, Cambridge CB2 0RE, England, UK 1"},{"name":"Department of Oncology, University of Cambridge, Cambridge CB2 3RA, England, UK 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John V.","family":"Kilmartin","sequence":"additional","affiliation":[{"name":"Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, England, UK 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanni","family":"Gergely","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Research Institute, Cambridge CB2 0RE, England, UK 1"},{"name":"Department of Oncology, University of Cambridge, Cambridge CB2 3RA, England, UK 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2010,4,5]]},"reference":[{"key":"2023072905171028600_bib1","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1038\/nature02166","article-title":"Proteomic characterization of the human centrosome by protein correlation profiling","volume":"426","author":"Andersen","year":"2003","journal-title":"Nature."},{"key":"2023072905171028600_bib2","doi-asserted-by":"publisher","first-page":"2987","DOI":"10.1242\/jcs.013136","article-title":"Aurora-A: the maker and breaker of spindle poles","volume":"120","author":"Barr","year":"2007","journal-title":"J. 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