{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:43:52Z","timestamp":1791251032715,"version":"4.1.0"},"reference-count":27,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,12,13]]},"abstract":"<jats:p>CDK5RAP2 is a human microcephaly protein that contains a \u03b3-tubulin complex (\u03b3-TuC)\u2013binding domain conserved in Drosophila melanogaster centrosomin and Schizosaccharomyces pombe Mto1p and Pcp1p, which are \u03b3-TuC\u2013tethering proteins. In this study, we show that this domain within CDK5RAP2 associates with the \u03b3-tubulin ring complex (\u03b3-TuRC) to stimulate its microtubule-nucleating activity and is therefore referred to as the \u03b3-TuRC\u2013mediated nucleation activator (\u03b3-TuNA). \u03b3-TuNA but not its \u03b3-TuC\u2013binding-deficient mutant stimulates microtubule nucleation by purified \u03b3-TuRC in vitro and induces extensive, \u03b3-TuRC-dependent nucleation of microtubules in a microtubule regrowth assay. \u03b3-TuRC bound to \u03b3-TuNA contains NME7, FAM128A\/B, and actin in addition to \u03b3-tubulin and GCP2\u20136. RNA interference\u2013mediated depletion of CDK5RAP2 impairs both centrosomal and acentrosomal microtubule nucleation, although \u03b3-TuRC assembly is unaffected. Collectively, these results suggest that the \u03b3-TuNA found in CDK5RAP2 has regulatory functions in \u03b3-TuRC\u2013mediated microtubule nucleation.<\/jats:p>","DOI":"10.1083\/jcb.201007030","type":"journal-article","created":{"date-parts":[[2010,12,7]],"date-time":"2010-12-07T01:20:37Z","timestamp":1291684837000},"page":"1089-1095","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":301,"title":["CDK5RAP2 stimulates microtubule nucleation by the \u03b3-tubulin ring complex"],"prefix":"10.1083","volume":"191","author":[{"given":"Yuk-Kwan","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"},{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"},{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"},{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siu Kwan","family":"Sze","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Nanyang Technological University, Singapore 637551 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"},{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Z.","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"},{"name":"Department of Biochemistry 1 , 2 , and 3"},{"name":"State Key Laboratory of Molecular Neuroscience 1 , 2 , and 3"},{"name":"Nanoscience and Nanotechnology Program, The Hong Kong University of Science and Technology, Hong Kong, China 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2010,12,6]]},"reference":[{"key":"2023072302045965900_bib1","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1038\/ng1539","article-title":"A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain size","volume":"37","author":"Bond","year":"2005","journal-title":"Nat. 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