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To further characterize the extent of perturbation induced by HIV-1, we performed proteomics analyses of nuclear envelopes (NEs) isolated from infected T cells. Infection induced extensive changes in the composition of the NE and its associated proteins, including a remarkable decrease in the abundance of Nups. Immunogold electron microscopy revealed the translocation of Nups into the cytoplasm. Nup62 was identified as a component of purified virus, and small interfering RNA depletion studies revealed an important role for this Nup in virus gene expression and infectivity. This detailed analysis highlights the profound effects on NE composition induced by HIV-1 infection, providing further evidence of the magnitude of viral control over the cell biology of its host.<\/jats:p>","DOI":"10.1083\/jcb.201008064","type":"journal-article","created":{"date-parts":[[2011,5,16]],"date-time":"2011-05-16T18:03:51Z","timestamp":1305569031000},"page":"619-631","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":76,"title":["HIV-1 remodels the nuclear pore complex"],"prefix":"10.1083","volume":"193","author":[{"given":"Anne","family":"Monette","sequence":"first","affiliation":[{"name":"HIV-1 RNA Trafficking Laboratory, Lady Davis Institute for Medical Research, Sir Mortimer B. 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