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Moreover, we show that MIB1 is sufficient to activate Wnt\/\u03b2-catenin (CTNNB1) signaling and that this activity depends on endogenous RYK. Conversely, in loss-of-function studies, both RYK and MIB1 are required for Wnt-3A\u2013mediated activation of CTNNB1. Finally, we identify the Caenorhabditis elegans orthologue of MIB1 and demonstrate a genetic interaction between ceMIB and lin-18\/RYK in vulva development. These findings provide insights into the mechanisms of Wnt\/RYK signaling and point to novel targets for the modulation of Wnt signaling.<\/jats:p>","DOI":"10.1083\/jcb.201107021","type":"journal-article","created":{"date-parts":[[2011,8,30]],"date-time":"2011-08-30T00:47:02Z","timestamp":1314665222000},"page":"737-750","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":99,"title":["Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt\/\u03b2-catenin signaling"],"prefix":"10.1083","volume":"194","author":[{"given":"Jason D.","family":"Berndt","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Aoyagi","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"},{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peitzu","family":"Yang","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"},{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie N.","family":"Anastas","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"},{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"},{"name":"Molecular and Cellular Biology graduate program, University of Washington, Seattle, WA 98109 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Tang","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"},{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randall T.","family":"Moon","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"},{"name":"Howard Hughes Medical Institute, Department of Pharmacology 1 , and 2"},{"name":"Institute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, WA 98109 1 , and 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2011,8,29]]},"reference":[{"key":"2023072302493893700_bib1","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1038\/nrm2717","article-title":"Proximal events in Wnt signal transduction","volume":"10","author":"Angers","year":"2009","journal-title":"Nat. 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