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This new toxin conjugate enabled us to conduct a genetic screen in human cells, which identified ST3GAL5, SLC35A2, B3GALT4, UGCG, and ELF4 as genes essential for CTx intoxication. The first four encode proteins involved in the synthesis of gangliosides, which are known receptors for CTx. Identification and isolation of the ST3GAL5 and SLC35A2 mutant clonal cells uncover a previously unappreciated differential contribution of gangliosides to intoxication by CTx.<\/jats:p>","DOI":"10.1083\/jcb.201108103","type":"journal-article","created":{"date-parts":[[2011,11,28]],"date-time":"2011-11-28T18:40:06Z","timestamp":1322505606000},"page":"751-764","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":65,"title":["Identification of host cell factors required for intoxication through use of modified cholera toxin"],"prefix":"10.1083","volume":"195","author":[{"given":"Carla P.","family":"Guimaraes","sequence":"first","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan E.","family":"Carette","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malini","family":"Varadarajan","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Antos","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian W.","family":"Popp","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Spooner","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thijn R.","family":"Brummelkamp","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidde L.","family":"Ploegh","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research, Cambridge, MA 02142 1"},{"name":"Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2011,11,28]]},"reference":[{"key":"2023072303101441000_bib1","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.1073\/pnas.83.5.1320","article-title":"Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution","volume":"83","author":"Allured","year":"1986","journal-title":"Proc. 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