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We used EBV to investigate the link between zones of pre-replication complex (pre-RC) assembly, replication initiation, and micrococcal nuclease (MNase) sensitivity at different cell cycle stages in a genome-wide fashion. The dyad symmetry element (DS) of EBV\u2019s latent origin, a well-established and very efficient pre-RC assembly region, served as an internal control. We identified 64 pre-RC zones that correlate spatially with 57 short nascent strand (SNS) zones. MNase experiments revealed that pre-RC and SNS zones were linked to regions of increased MNase sensitivity, which is a marker of origin strength. Interestingly, although spatially correlated, pre-RC and SNS zones were characterized by different features. We propose that pre-RCs are formed at flexible but distinct sites, from which only a few are activated per single genome and cell cycle.<\/jats:p>","DOI":"10.1083\/jcb.201109105","type":"journal-article","created":{"date-parts":[[2012,8,14]],"date-time":"2012-08-14T01:48:11Z","timestamp":1344908891000},"page":"509-528","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":17,"title":["Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus"],"prefix":"10.1083","volume":"198","author":[{"given":"Peer","family":"Papior","sequence":"first","affiliation":[{"name":"DNA Replication and Epigenetics group, Research Unit Gene Vectors, Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health, 81377 M\u00fcnchen, Germany 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"Arteaga-Salas","sequence":"additional","affiliation":[{"name":"Adolf Butenandt Institut, Ludwig Maximilians University, 80336 M\u00fcnchen Germany 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"G\u00fcnther","sequence":"additional","affiliation":[{"name":"Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 20251 Hamburg Germany 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Grundhoff","sequence":"additional","affiliation":[{"name":"Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 20251 Hamburg Germany 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aloys","family":"Schepers","sequence":"additional","affiliation":[{"name":"DNA Replication and Epigenetics group, Research Unit Gene Vectors, Helmholtz Zentrum M\u00fcnchen, German Research Center for Environmental Health, 81377 M\u00fcnchen, Germany 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2012,8,13]]},"reference":[{"key":"2023072302390962000_bib1","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1128\/jvi.61.5.1743-1746.1987","article-title":"Replication of latent Epstein-Barr virus genomes in Raji cells","volume":"61","author":"Adams","year":"1987","journal-title":"J. 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