{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:42:44Z","timestamp":1791423764486,"version":"4.3.3"},"reference-count":41,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,8,20]]},"abstract":"<jats:p>Human and murine platelets (PLTs) variably express toll-like receptors (TLRs), which link the innate and adaptive immune responses during infectious inflammation and atherosclerotic vascular disease. In this paper, we show that the TLR9 transcript is specifically up-regulated during pro-PLT production and is distributed to a novel electron-dense tubular system-related compartment we have named the T granule. TLR9 colocalizes with protein disulfide isomerase and is associated with either VAMP 7 or VAMP 8, which regulates its distribution in PLTs on contact activation (spreading). Preincubation of PLTs with type IV collagen specifically increased TLR9 and CD62P surface expression and augmented oligodeoxynucleotide (ODN) sequestration and PLT clumping upon addition of bacterial\/viral ODNs. Collectively, this paper (a) tracks TLR9 to a new intracellular compartment in PLTs and (b) describes a novel mechanism of TLR9 organization and signaling in human PLTs.<\/jats:p>","DOI":"10.1083\/jcb.201111136","type":"journal-article","created":{"date-parts":[[2012,8,20]],"date-time":"2012-08-20T11:34:13Z","timestamp":1345462453000},"page":"561-574","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":189,"title":["T granules in human platelets function in TLR9 organization and signaling"],"prefix":"10.1083","volume":"198","author":[{"given":"Jonathan N.","family":"Thon","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA 02115 1"},{"name":"Deparment of Medicine 2 and 3"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115 2 and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher G.","family":"Peters","sequence":"additional","affiliation":[{"name":"Deparment of Medicine 2 and 3"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115 2 and 3"},{"name":"Immune Disease Institute, Boston, MA 02115 6"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kellie R.","family":"Machlus","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA 02115 1"},{"name":"Deparment of Medicine 2 and 3"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115 2 and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rukhsana","family":"Aslam","sequence":"additional","affiliation":[{"name":"Toronto Platelet Immunobiology Group, Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael\u2019s Hospital, Toronto, Ontario M5B-1W8, Canada 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse","family":"Rowley","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Utah, Salt Lake City, UT 84112 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Macleod","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA 02115 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"Devine","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA 02115 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias A.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Deparment of Medicine 2 and 3"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115 2 and 3"},{"name":"Immune Disease Institute, Boston, MA 02115 6"},{"name":"Program in Cellular and Molecular Medicine, Children\u2019s Hospital, Boston, MA 02115 7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew S.","family":"Weyrich","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Utah, Salt Lake City, UT 84112 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Semple","sequence":"additional","affiliation":[{"name":"Toronto Platelet Immunobiology Group, Keenan Research Centre, Li Ka Shing Knowledge Institute, St. Michael\u2019s Hospital, Toronto, Ontario M5B-1W8, Canada 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Flaumenhaft","sequence":"additional","affiliation":[{"name":"Deparment of Medicine 2 and 3"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115 2 and 3"},{"name":"Division of Hemostasis and Thrombosis, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02115 8"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Joseph E.","family":"Italiano","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA 02115 1"},{"name":"Deparment of Medicine 2 and 3"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115 2 and 3"},{"name":"Vascular Biology Program, Department of Surgery, Children\u2019s Hospital, Boston, MA 02115 9"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2012,8,20]]},"reference":[{"key":"2023072302354597200_bib1","doi-asserted-by":"publisher","first-page":"13932","DOI":"10.1073\/pnas.0606411103","article-title":"Disulfide isomerization switches tissue factor from coagulation to cell signaling","volume":"103","author":"Ahamed","year":"2006","journal-title":"Proc. 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