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Although a role for N-WASP in invadopodia was known, we now show how N-WASP regulates invasive protrusion in 3D matrices. In actively invading cells, N-WASP promoted trafficking of MT1-MMP into invasive pseudopodia, primarily from late endosomes, from which it was delivered to the plasma membrane. Upon MT1-MMP\u2019s arrival at the plasma membrane in pseudopodia, N-WASP stabilized MT1-MMP via direct tethering of its cytoplasmic tail to F-actin. Thus, N-WASP is crucial for extension of invasive pseudopods into which MT1-MMP traffics and for providing the correct cytoskeletal framework to couple matrix remodeling with protrusive invasion.<\/jats:p>","DOI":"10.1083\/jcb.201203025","type":"journal-article","created":{"date-parts":[[2012,10,23]],"date-time":"2012-10-23T20:29:50Z","timestamp":1351024190000},"page":"527-544","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":163,"title":["N-WASP coordinates the delivery and F-actin\u2013mediated capture of MT1-MMP at invasive pseudopods"],"prefix":"10.1083","volume":"199","author":[{"given":"Xinzi","family":"Yu","sequence":"first","affiliation":[{"name":"The Beatson Institute for Cancer Research, Bearsden, Glasgow G61 1BD, Scotland, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Zech","sequence":"additional","affiliation":[{"name":"The Beatson Institute for Cancer Research, Bearsden, 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