{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:12:34Z","timestamp":1791295954968,"version":"4.1.0"},"reference-count":40,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,6,10]]},"abstract":"<jats:p>Protein modifications by ubiquitin and small ubiquitin-like modifier (SUMO) play key roles in cellular signaling pathways. SUMO-targeted ubiquitin ligases (STUbLs) directly couple these modifications by selectively recognizing SUMOylated target proteins through SUMO-interacting motifs (SIMs), promoting their K48-linked ubiquitylation and degradation. Only a single mammalian STUbL, RNF4, has been identified. We show that human RNF111\/Arkadia is a new STUbL, which used three adjacent SIMs for specific recognition of poly-SUMO2\/3 chains, and used Ubc13\u2013Mms2 as a cognate E2 enzyme to promote nonproteolytic, K63-linked ubiquitylation of SUMOylated target proteins. We demonstrate that RNF111 promoted ubiquitylation of SUMOylated XPC (xeroderma pigmentosum C) protein, a central DNA damage recognition factor in nucleotide excision repair (NER) extensively regulated by ultraviolet (UV)-induced SUMOylation and ubiquitylation. Moreover, we show that RNF111 facilitated NER by regulating the recruitment of XPC to UV-damaged DNA. Our findings establish RNF111 as a new STUbL that directly links nonproteolytic ubiquitylation and SUMOylation in the DNA damage response.<\/jats:p>","DOI":"10.1083\/jcb.201212075","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T15:18:59Z","timestamp":1370877539000},"page":"797-807","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":159,"title":["RNF111\/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response"],"prefix":"10.1083","volume":"201","author":[{"given":"Sara L.","family":"Poulsen","sequence":"first","affiliation":[{"name":"Ubiquitin Signaling Group, Department of Disease Biology 1 , 2 , and 3"},{"name":"Department of Proteomics 1 , 2 , and 3"},{"name":"Protein Function and Interactions Group, The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca K.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Ubiquitin Signaling Group, Department of Disease Biology 1 , 2 , and 3"},{"name":"Department of Proteomics 1 , 2 , and 3"},{"name":"Protein Function and Interactions Group, The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian A.","family":"Wagner","sequence":"additional","affiliation":[{"name":"Ubiquitin Signaling Group, Department of Disease Biology 1 , 2 , and 3"},{"name":"Department of Proteomics 1 , 2 , and 3"},{"name":"Protein Function and Interactions Group, The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loes","family":"van 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3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Wikstr\u00f6m","sequence":"additional","affiliation":[{"name":"Ubiquitin Signaling Group, Department of Disease Biology 1 , 2 , and 3"},{"name":"Department of Proteomics 1 , 2 , and 3"},{"name":"Protein Function and Interactions Group, The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunaram","family":"Choudhary","sequence":"additional","affiliation":[{"name":"Ubiquitin Signaling Group, Department of Disease Biology 1 , 2 , and 3"},{"name":"Department of Proteomics 1 , 2 , and 3"},{"name":"Protein Function and Interactions Group, The Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, DK-2200 Copenhagen, Denmark 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriaan 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