{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:59:41Z","timestamp":1791187181292,"version":"4.1.0"},"reference-count":52,"publisher":"Rockefeller University Press","issue":"6","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,6,10]]},"abstract":"<jats:p>Though critical to normal development and cancer metastasis, how cells traverse basement membranes is poorly understood. A central impediment has been the challenge of visualizing invasive cell interactions with basement membrane in vivo. By developing live-cell imaging methods to follow anchor cell (AC) invasion in Caenorhabditis elegans, we identify F-actin\u2013based invadopodia that breach basement membrane. When an invadopodium penetrates basement membrane, it rapidly transitions into a stable invasive process that expands the breach and crosses into the vulval tissue. We find that the netrin receptor UNC-40 (DCC) specifically enriches at the site of basement membrane breach and that activation by UNC-6 (netrin) directs focused F-actin formation, generating the invasive protrusion and the cessation of invadopodia. Using optical highlighting of basement membrane components, we further demonstrate that rather than relying solely on proteolytic dissolution, the AC\u2019s protrusion physically displaces basement membrane. These studies reveal an UNC-40\u2013mediated morphogenetic transition at the cell\u2013basement membrane interface that directs invading cells across basement membrane barriers.<\/jats:p>","DOI":"10.1083\/jcb.201301091","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T11:18:59Z","timestamp":1370863139000},"page":"903-913","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":127,"title":["The netrin receptor DCC focuses invadopodia-driven basement membrane transmigration in vivo"],"prefix":"10.1083","volume":"201","author":[{"given":"Elliott J.","family":"Hagedorn","sequence":"first","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua W.","family":"Ziel","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan A.","family":"Morrissey","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara M.","family":"Linden","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyi","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sam A.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"},{"name":"Light Microscopy Core Facility, Duke University and Duke University Medical Center, Durham, NC 27708 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R.","family":"Sherwood","sequence":"additional","affiliation":[{"name":"Department of Biology, Duke University, Durham, NC 27708 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2013,6,10]]},"reference":[{"key":"2023072305593804600_bib1","doi-asserted-by":"publisher","first-page":"3034","DOI":"10.1158\/0008-5472.CAN-05-2177","article-title":"Dynamic interactions of cortactin and membrane type 1 matrix metalloproteinase at invadopodia: defining the stages of invadopodia formation and function","volume":"66","author":"Artym","year":"2006","journal-title":"Cancer Res."},{"key":"2023072305593804600_bib2","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1242\/jcs.008037","article-title":"Multiple regulatory inputs converge on cortactin to control invadopodia biogenesis and extracellular matrix degradation","volume":"121","author":"Ayala","year":"2008","journal-title":"J. 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