{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:00:54Z","timestamp":1791291654381,"version":"4.1.0"},"reference-count":41,"publisher":"Rockefeller University Press","issue":"2","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,4,28]]},"abstract":"<jats:p>Insights into mechanisms coordinating membrane remodeling, local actin nucleation, and postsynaptic scaffolding during postsynapse formation are important for understanding vertebrate brain function. Gene knockout and RNAi in individual neurons reveal that the F-BAR protein syndapin I is a crucial postsynaptic coordinator in formation of excitatory synapses. Syndapin I deficiency caused significant reductions of synapse and dendritic spine densities. These syndapin I functions reflected direct, SH3 domain\u2013mediated associations and functional interactions with ProSAP1\/Shank2. They furthermore required F-BAR domain-mediated membrane binding. Ultra-high-resolution imaging of specifically membrane-associated, endogenous syndapin I at membranes of freeze-fractured neurons revealed that membrane-bound syndapin I preferentially occurred in spines and formed clusters at distinct postsynaptic membrane subareas. Postsynaptic syndapin I deficiency led to reduced frequencies of miniature excitatory postsynaptic currents, i.e., to defects in synaptic transmission phenocopying ProSAP1\/Shank2 knockout, and impairments in proper synaptic ProSAP1\/Shank2 distribution. Syndapin I\u2013enriched membrane nanodomains thus seem to be important spatial cues and organizing platforms, shaping dendritic membrane areas into synaptic compartments.<\/jats:p>","DOI":"10.1083\/jcb.201307088","type":"journal-article","created":{"date-parts":[[2014,4,22]],"date-time":"2014-04-22T03:46:52Z","timestamp":1398138412000},"page":"197-215","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":47,"title":["ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function"],"prefix":"10.1083","volume":"205","author":[{"given":"Katharina","family":"Schneider","sequence":"first","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Seemann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Liebmann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi","family":"Ahuja","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis","family":"Koch","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Westermann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian A.","family":"H\u00fcbner","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael M.","family":"Kessels","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britta","family":"Qualmann","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry I 1 , 2 , and 3"},{"name":"Institute for Human Genetics 1 , 2 , and 3"},{"name":"Electron Microscopy Center, Jena University Hospital, Friedrich Schiller University Jena, 07743 Jena, Germany 1 , 2 , and 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2014,4,21]]},"reference":[{"key":"2023072306342529400_bib1","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/j.cell.2007.08.030","article-title":"Cordon-bleu is an actin nucleation factor and controls neuronal morphology","volume":"131","author":"Ahuja","year":"2007","journal-title":"Cell."},{"key":"2023072306342529400_bib2","doi-asserted-by":"publisher","first-page":"3925","DOI":"10.1523\/JNEUROSCI.1754-07.2008","article-title":"Perturbation of syndapin\/PACSIN impairs synaptic vesicle recycling evoked by intense stimulation","volume":"28","author":"Andersson","year":"2008","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib3","doi-asserted-by":"publisher","first-page":"752","DOI":"10.1038\/nn1695","article-title":"Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis","volume":"9","author":"Anggono","year":"2006","journal-title":"Nat. Neurosci."},{"key":"2023072306342529400_bib4","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1093\/hmg\/ddr470","article-title":"Inherited and de novo SHANK2 variants associated with autism spectrum disorder impair neuronal morphogenesis and physiology","volume":"21","author":"Berkel","year":"2012","journal-title":"Hum. Mol. Genet."},{"key":"2023072306342529400_bib5","doi-asserted-by":"crossref","first-page":"6506","DOI":"10.1523\/JNEUROSCI.19-15-06506.1999","article-title":"Proline-rich synapse-associated protein-1\/cortactin binding protein 1 (ProSAP1\/CortBP1) is a PDZ-domain protein highly enriched in the postsynaptic density","volume":"19","author":"Boeckers","year":"1999","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib6","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1006\/bbrc.1999.1489","article-title":"Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP\/GKAP family","volume":"264","author":"Boeckers","year":"1999","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2023072306342529400_bib7","doi-asserted-by":"publisher","first-page":"3642","DOI":"10.1091\/mbc.E05-01-0076","article-title":"EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling","volume":"16","author":"Braun","year":"2005","journal-title":"Mol. Biol. Cell."},{"key":"2023072306342529400_bib8","doi-asserted-by":"publisher","first-page":"2447","DOI":"10.1523\/JNEUROSCI.4433-10.2011","article-title":"WRP\/srGAP3 facilitates the initiation of spine development by an inverse F-BAR domain, and its loss impairs long-term memory","volume":"31","author":"Carlson","year":"2011","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib9","doi-asserted-by":"publisher","first-page":"13315","DOI":"10.1523\/JNEUROSCI.3973-09.2009","article-title":"F-BAR proteins of the syndapin family shape the plasma membrane and are crucial for neuromorphogenesis","volume":"29","author":"Dharmalingam","year":"2009","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib10","doi-asserted-by":"crossref","first-page":"3443","DOI":"10.1242\/jcs.108.11.3443","article-title":"Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes","volume":"108","author":"Fujimoto","year":"1995","journal-title":"J. Cell Sci."},{"key":"2023072306342529400_bib11","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1038\/emboj.2010.336","article-title":"Concerted action of zinc and ProSAP\/Shank in synaptogenesis and synapse maturation","volume":"30","author":"Grabrucker","year":"2011","journal-title":"EMBO J."},{"key":"2023072306342529400_bib12","doi-asserted-by":"publisher","first-page":"17842","DOI":"10.1523\/JNEUROSCI.0843-12.2012","article-title":"The actin nucleator Cobl is crucial for Purkinje cell development and works in close conjunction with the F-actin binding protein Abp1","volume":"32","author":"Haag","year":"2012","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib13","doi-asserted-by":"publisher","first-page":"10031","DOI":"10.1523\/JNEUROSCI.0336-08.2008","article-title":"The actin-binding protein Abp1 controls dendritic spine morphology and is important for spine head and synapse formation","volume":"28","author":"Haeckel","year":"2008","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib14","doi-asserted-by":"crossref","first-page":"11759","DOI":"10.1523\/JNEUROSCI.23-37-11759.2003","article-title":"Activity-dependent redistribution and essential role of cortactin in dendritic spine morphogenesis","volume":"23","author":"Hering","year":"2003","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib15","doi-asserted-by":"publisher","first-page":"1697","DOI":"10.1523\/JNEUROSCI.3032-07.2008","article-title":"Smaller dendritic spines, weaker synaptic transmission, but enhanced spatial learning in mice lacking Shank1","volume":"28","author":"Hung","year":"2008","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib16","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1016\/j.devcel.2005.11.005","article-title":"Dynamin and the actin cytoskeleton cooperatively regulate plasma membrane invagination by BAR and F-BAR proteins","volume":"9","author":"Itoh","year":"2005","journal-title":"Dev. Cell."},{"key":"2023072306342529400_bib17","doi-asserted-by":"publisher","first-page":"6083","DOI":"10.1093\/emboj\/cdf604","article-title":"Syndapins integrate N-WASP in receptor-mediated endocytosis","volume":"21","author":"Kessels","year":"2002","journal-title":"EMBO J."},{"key":"2023072306342529400_bib18","doi-asserted-by":"publisher","first-page":"13285","DOI":"10.1074\/jbc.M510226200","article-title":"Syndapin oligomers interconnect the machineries for endocytic vesicle formation and actin polymerization","volume":"281","author":"Kessels","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2023072306342529400_bib19","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1091\/mbc.11.1.393","article-title":"Association of mouse actin-binding protein 1 (mAbp1\/SH3P7), an Src kinase target, with dynamic regions of the cortical actin cytoskeleton in response to Rac1 activation","volume":"11","author":"Kessels","year":"2000","journal-title":"Mol. Biol. Cell."},{"key":"2023072306342529400_bib20","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1083\/jcb.153.2.351","article-title":"Mammalian Abp1, a signal-responsive F-actin-binding protein, links the actin cytoskeleton to endocytosis via the GTPase dynamin","volume":"153","author":"Kessels","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072306342529400_bib21","doi-asserted-by":"publisher","first-page":"4955","DOI":"10.1038\/emboj.2011.339","article-title":"Proper synaptic vesicle formation and neuronal network activity critically rely on syndapin I","volume":"30","author":"Koch","year":"2011","journal-title":"EMBO J."},{"key":"2023072306342529400_bib22","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1007\/s00418-012-0945-0","article-title":"Ultrastructural freeze-fracture immunolabeling identifies plasma membrane-localized syndapin II as a crucial factor in shaping caveolae","volume":"138","author":"Koch","year":"2012","journal-title":"Histochem. Cell Biol."},{"key":"2023072306342529400_bib23","doi-asserted-by":"publisher","first-page":"3578","DOI":"10.1242\/jcs.101451","article-title":"Abp1 utilizes the Arp2\/3 complex activator Scar\/WAVE in bristle development","volume":"125","author":"Koch","year":"2012","journal-title":"J. Cell Sci."},{"key":"2023072306342529400_bib24","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1091\/mbc.E09-07-0596","article-title":"Molecular architecture of synaptic actin cytoskeleton in hippocampal neurons reveals a mechanism of dendritic spine morphogenesis","volume":"21","author":"Korobova","year":"2010","journal-title":"Mol. Biol. Cell."},{"key":"2023072306342529400_bib25","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1038\/nature09965","article-title":"Shank3 mutant mice display autistic-like behaviours and striatal dysfunction","volume":"472","author":"Pe\u00e7a","year":"2011","journal-title":"Nature."},{"key":"2023072306342529400_bib26","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1038\/nn1680","article-title":"Endocytosis and synaptic removal of NR3A-containing NMDA receptors by PACSIN1\/syndapin1","volume":"9","author":"P\u00e9rez-Ota\u00f1o","year":"2006","journal-title":"Nat. Neurosci."},{"key":"2023072306342529400_bib27","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1083\/jcb.148.5.1047","article-title":"Syndapin isoforms participate in receptor-mediated endocytosis and actin organization","volume":"148","author":"Qualmann","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023072306342529400_bib28","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1091\/mbc.10.2.501","article-title":"Syndapin I, a synaptic dynamin-binding protein that associates with the neural Wiskott-Aldrich syndrome protein","volume":"10","author":"Qualmann","year":"1999","journal-title":"Mol. Biol. Cell."},{"key":"2023072306342529400_bib29","doi-asserted-by":"publisher","first-page":"2481","DOI":"10.1523\/JNEUROSCI.5479-03.2004","article-title":"Linkage of the actin cytoskeleton to the postsynaptic density via direct interactions of Abp1 with the ProSAP\/Shank family","volume":"24","author":"Qualmann","year":"2004","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib30","doi-asserted-by":"publisher","first-page":"3501","DOI":"10.1038\/emboj.2011.266","article-title":"Let\u2019s go bananas: revisiting the endocytic BAR code","volume":"30","author":"Qualmann","year":"2011","journal-title":"EMBO J."},{"key":"2023072306342529400_bib31","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1111\/j.1365-2818.1990.tb03008.x","article-title":"Cooling rate and ice-crystal measurement in biological specimens plunged into liquid ethane, propane, and Freon 22","volume":"158","author":"Ryan","year":"1990","journal-title":"J. Microsc."},{"key":"2023072306342529400_bib32","doi-asserted-by":"publisher","first-page":"9303","DOI":"10.1074\/jbc.M113.461293","article-title":"Casein kinase 2 phosphorylation of protein kinase C and casein kinase 2 substrate in neurons (PACSIN) 1 protein regulates neuronal spine formation","volume":"288","author":"Schael","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2023072306342529400_bib33","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/nature11015","article-title":"Autistic-like behaviours and hyperactivity in mice lacking ProSAP1\/Shank2","volume":"486","author":"Schmeisser","year":"2012","journal-title":"Nature."},{"key":"2023072306342529400_bib34","doi-asserted-by":"publisher","first-page":"196","DOI":"10.1242\/jcs.111674","article-title":"Ciliated sensory hair cell formation and function require the F-BAR protein syndapin I and the WH2 domain-based actin nucleator Cobl","volume":"126","author":"Sch\u00fcler","year":"2013","journal-title":"J. Cell Sci."},{"key":"2023072306342529400_bib35","doi-asserted-by":"publisher","first-page":"3147","DOI":"10.1038\/emboj.2011.207","article-title":"The functions of the actin nucleator Cobl in cellular morphogenesis critically depend on syndapin I","volume":"30","author":"Schwintzer","year":"2011","journal-title":"EMBO J."},{"key":"2023072306342529400_bib36","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/S0091-679X(08)00411-1","article-title":"Freeze-fracture cytochemistry in cell biology","volume":"88","author":"Severs","year":"2008","journal-title":"Methods Cell Biol."},{"key":"2023072306342529400_bib37","doi-asserted-by":"publisher","first-page":"7300","DOI":"10.1523\/JNEUROSCI.0269-11.2011","article-title":"Synaptic glutamate release is modulated by the Na+-driven Cl-\/HCO3- exchanger Slc4a8","volume":"31","author":"Sinning","year":"2011","journal-title":"J. Neurosci."},{"key":"2023072306342529400_bib38","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.conb.2005.12.001","article-title":"Molecular mechanisms of dendritic spine morphogenesis","volume":"16","author":"Tada","year":"2006","journal-title":"Curr. Opin. Neurobiol."},{"key":"2023072306342529400_bib39","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1083\/jcb.142.2.499","article-title":"Bassoon, a novel zinc-finger CAG\/glutamine-repeat protein selectively localized at the active zone of presynaptic nerve terminals","volume":"142","author":"tom Dieck","year":"1998","journal-title":"J. Cell Biol."},{"key":"2023072306342529400_bib40","doi-asserted-by":"publisher","first-page":"12700","DOI":"10.1073\/pnas.0902974106","article-title":"Molecular mechanism of membrane constriction and tubulation mediated by the F-BAR protein Pacsin\/Syndapin","volume":"106","author":"Wang","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072306342529400_bib41","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1038\/nature11208","article-title":"Autistic-like social behaviour in Shank2-mutant mice improved by restoring NMDA receptor function","volume":"486","author":"Won","year":"2012","journal-title":"Nature."}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/205\/2\/197\/1583489\/jcb_201307088.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/205\/2\/197\/1583489\/jcb_201307088.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,23]],"date-time":"2023-07-23T06:36:19Z","timestamp":1690094179000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/205\/2\/197\/37650\/ProSAP1-and-membrane-nanodomain-associated"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,21]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,4,28]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.201307088","relation":{},"ISSN":["1540-8140","0021-9525"],"issn-type":[{"value":"1540-8140","type":"electronic"},{"value":"0021-9525","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4,21]]}}}