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Constrained ICAM-1 mobility is important for DC function, as DCs expressing a high-mobility ICAM-1 mutant lacking the cytoplasmic domain exhibit diminished antigen-dependent conjugate formation and T cell priming. These defects are associated with inefficient induction of leukocyte functional antigen 1 (LFA-1) affinity maturation, which is consistent with a model in which constrained ICAM-1 mobility opposes forces on LFA-1 exerted by the T cell cytoskeleton, whereas ICAM-1 clustering enhances valency and further promotes ligand-dependent LFA-1 activation. Our results reveal an important new mechanism through which the DC cytoskeleton regulates receptor activation at the immunological synapse.<\/jats:p>","DOI":"10.1083\/jcb.201406120","type":"journal-article","created":{"date-parts":[[2015,2,10]],"date-time":"2015-02-10T01:10:46Z","timestamp":1423530646000},"page":"457-473","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":175,"title":["The dendritic cell cytoskeleton promotes T cell adhesion and activation by constraining ICAM-1 mobility"],"prefix":"10.1083","volume":"208","author":[{"given":"William A.","family":"Comrie","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia and Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuixing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia and Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Boyle","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia and Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis K.","family":"Burkhardt","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Children\u2019s Hospital of Philadelphia and Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA 19102"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,2,9]]},"reference":[{"key":"2023072307103400000_bib1","doi-asserted-by":"publisher","first-page":"1452","DOI":"10.4049\/jimmunol.166.3.1452","article-title":"The dendritic cell cytoskeleton is critical for the formation of the immunological synapse","volume":"166","author":"Al-Alwan","year":"2001","journal-title":"J. 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