{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:01:04Z","timestamp":1791874864854,"version":"4.3.4"},"reference-count":63,"publisher":"Rockefeller University Press","issue":"3","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,8,3]]},"abstract":"<jats:p>The ability to heal wounds efficiently is essential for life. After wounding of an epithelium, the cells bordering the wound form dynamic actin protrusions and\/or a contractile actomyosin cable, and these actin structures drive wound closure. Despite their importance in wound healing, the molecular mechanisms that regulate the assembly of these actin structures at wound edges are not well understood. In this paper, using Drosophila melanogaster embryos, we demonstrate that Diaphanous, SCAR, and WASp play distinct but overlapping roles in regulating actin assembly during wound healing. Moreover, we show that endocytosis is essential for wound edge actin assembly and wound closure. We identify adherens junctions (AJs) as a key target of endocytosis during wound healing and propose that endocytic remodeling of AJs is required to form \u201csignaling centers\u201d along the wound edge that control actin assembly. We conclude that coordination of actin assembly, AJ remodeling, and membrane traffic is required for the construction of a motile leading edge during wound healing.<\/jats:p>","DOI":"10.1083\/jcb.201411037","type":"journal-article","created":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T21:01:31Z","timestamp":1438030891000},"page":"419-433","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":38,"title":["Endocytosis-dependent coordination of multiple actin regulators is required for wound healing"],"prefix":"10.1083","volume":"210","author":[{"given":"Yutaka","family":"Matsubayashi","sequence":"first","affiliation":[{"name":"The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla","family":"Coulson-Gilmer","sequence":"additional","affiliation":[{"name":"The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom H.","family":"Millard","sequence":"additional","affiliation":[{"name":"The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, England, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,7,27]]},"reference":[{"key":"2023072308070869600_bib1","doi-asserted-by":"publisher","first-page":"5984","DOI":"10.1242\/jcs.109066","article-title":"Drosophila embryos close epithelial wounds using a combination of cellular protrusions and an actomyosin purse string","volume":"125","author":"Abreu-Blanco","year":"2012","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib2","doi-asserted-by":"crossref","first-page":"1887","DOI":"10.1242\/dev.127.9.1887","article-title":"Functional analysis of the Drosophila diaphanous FH protein in early embryonic development","volume":"127","author":"Afshar","year":"2000","journal-title":"Development."},{"key":"2023072308070869600_bib3","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1083\/jcb.201211039","article-title":"Coordinated waves of actomyosin flow and apical cell constriction immediately after wounding","volume":"202","author":"Antunes","year":"2013","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib4","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1083\/jcb.121.3.565","article-title":"A novel cytoskeletal structure involved in purse string wound closure and cell polarity maintenance","volume":"121","author":"Bement","year":"1993","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1083\/jcb.152.1.1","article-title":"Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell fate decisions mediated by Notch signaling","volume":"152","author":"Ben-Yaacov","year":"2001","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib6","first-page":"1382","article-title":"Microinjection techniques for studying mitosis in the Drosophila melanogaster syncytial embryo","volume":"31","author":"Brust-Mascher","year":"2009","journal-title":"J. Vis. Exp."},{"key":"2023072308070869600_bib7","doi-asserted-by":"publisher","first-page":"E5373","DOI":"10.1073\/pnas.1408224111","article-title":"The Toll\/NF-\u03baB signaling pathway is required for epidermal wound repair in Drosophila","volume":"111","author":"Carvalho","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"2023072308070869600_bib8","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1093\/genetics\/144.4.1673","article-title":"The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster","volume":"144","author":"Chou","year":"1996","journal-title":"Genetics."},{"key":"2023072308070869600_bib9","doi-asserted-by":"crossref","first-page":"3323","DOI":"10.1242\/jcs.111.22.3323","article-title":"Actin \u2018purse string\u2019 filaments are anchored by E-cadherin-mediated adherens junctions at the leading edge of the epithelial wound, providing coordinated cell movement","volume":"111","author":"Danjo","year":"1998","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib10","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1002\/gene.10113","article-title":"Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin","volume":"34","author":"Dutta","year":"2002","journal-title":"Genesis."},{"key":"2023072308070869600_bib11","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1038\/nature00859","article-title":"Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck","volume":"418","author":"Eden","year":"2002","journal-title":"Nature."},{"key":"2023072308070869600_bib12","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1034\/j.1600-0854.2002.030203.x","article-title":"Morphogen gradient formation and vesicular trafficking","volume":"3","author":"Entchev","year":"2002","journal-title":"Traffic."},{"key":"2023072308070869600_bib13","first-page":"1696","article-title":"Live imaging of Drosophila melanogaster embryonic hemocyte migrations","volume":"36","author":"Evans","year":"2010","journal-title":"J. Vis. Exp."},{"key":"2023072308070869600_bib14","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1016\/S0960-9822(00)00579-0","article-title":"Signaling pathways and cell mechanics involved in wound closure by epithelial cell sheets","volume":"10","author":"Fenteany","year":"2000","journal-title":"Curr. Biol."},{"key":"2023072308070869600_bib15","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1038\/nrm3266","article-title":"Dynamin, a membrane-remodelling GTPase","volume":"13","author":"Ferguson","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072308070869600_bib16","doi-asserted-by":"publisher","first-page":"3227","DOI":"10.1091\/mbc.E13-05-0228","article-title":"Wounded cells drive rapid epidermal repair in the early Drosophila embryo","volume":"24","author":"Fernandez-Gonzalez","year":"2013","journal-title":"Mol. Biol. Cell."},{"key":"2023072308070869600_bib17","doi-asserted-by":"publisher","first-page":"2208","DOI":"10.1016\/j.cub.2005.11.064","article-title":"Nonmuscle myosin II generates forces that transmit tension and drive contraction in multiple tissues during dorsal closure","volume":"15","author":"Franke","year":"2005","journal-title":"Curr. Biol."},{"key":"2023072308070869600_bib18","doi-asserted-by":"publisher","first-page":"e28349","DOI":"10.1371\/journal.pone.0028349","article-title":"Hole-in-one mutant phenotypes link EGFR\/ERK signaling to epithelial tissue repair in Drosophila","volume":"6","author":"Geiger","year":"2011","journal-title":"PLoS ONE."},{"key":"2023072308070869600_bib19","doi-asserted-by":"publisher","first-page":"1631","DOI":"10.1016\/j.cub.2008.09.029","article-title":"Cdc42, Par6, and aPKC regulate Arp2\/3-mediated endocytosis to control local adherens junction stability","volume":"18","author":"Georgiou","year":"2008","journal-title":"Curr. Biol."},{"key":"2023072308070869600_bib20","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/B978-0-12-394304-0.00007-5","article-title":"Adherens junction assembly and function in the Drosophila embryo","volume":"293","author":"Harris","year":"2012","journal-title":"Int Rev Cell Mol Biol."},{"key":"2023072308070869600_bib21","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1242\/dev.016337","article-title":"Diaphanous regulates myosin and adherens junctions to control cell contractility and protrusive behavior during morphogenesis","volume":"135","author":"Homem","year":"2008","journal-title":"Development."},{"key":"2023072308070869600_bib22","doi-asserted-by":"publisher","first-page":"5138","DOI":"10.1091\/mbc.E09-02-0144","article-title":"Exploring the roles of diaphanous and enabled activity in shaping the balance between filopodia and lamellipodia","volume":"20","author":"Homem","year":"2009","journal-title":"Mol. Biol. Cell."},{"key":"2023072308070869600_bib23","doi-asserted-by":"publisher","first-page":"939","DOI":"10.1083\/jcb.200510043","article-title":"Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells","volume":"171","author":"Ikenouchi","year":"2005","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib24","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.jtbi.2012.08.017","article-title":"Bayesian inference of force dynamics during morphogenesis","volume":"313","author":"Ishihara","year":"2012","journal-title":"J. Theor. Biol."},{"key":"2023072308070869600_bib25","doi-asserted-by":"publisher","first-page":"1420","DOI":"10.1016\/S0960-9822(00)00796-X","article-title":"Dynamic actin-based epithelial adhesion and cell matching during Drosophila dorsal closure","volume":"10","author":"Jacinto","year":"2000","journal-title":"Curr. Biol."},{"key":"2023072308070869600_bib26","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1083\/jcb.149.2.471","article-title":"Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila.","volume":"149","author":"Kiehart","year":"2000","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3109\/01677069009084149","article-title":"Allelic interactions at the shibire locus of Drosophila: effects on behavior","volume":"7","author":"Kim","year":"1990","journal-title":"J. Neurogenet."},{"key":"2023072308070869600_bib28","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1038\/nrm3861","article-title":"Steering cell migration: lamellipodium dynamics and the regulation of directional persistence","volume":"15","author":"Krause","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072308070869600_bib29","doi-asserted-by":"publisher","first-page":"1867","DOI":"10.1016\/j.cub.2003.10.005","article-title":"Abi, Sra1, and Kette control the stability and localization of SCAR\/WAVE to regulate the formation of actin-based protrusions","volume":"13","author":"Kunda","year":"2003","journal-title":"Curr. Biol."},{"key":"2023072308070869600_bib30","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1016\/S0896-6273(00)80701-1","article-title":"Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis","volume":"22","author":"Lee","year":"1999","journal-title":"Neuron."},{"key":"2023072308070869600_bib31","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1016\/j.cub.2008.09.063","article-title":"Drosophila Cip4 and WASp define a branch of the Cdc42-Par6-aPKC pathway regulating E-cadherin endocytosis","volume":"18","author":"Leibfried","year":"2008","journal-title":"Curr. Biol."},{"key":"2023072308070869600_bib32","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1038\/ncb2224","article-title":"Spatial regulation of Dia and Myosin-II by RhoGEF2 controls initiation of E-cadherin endocytosis during epithelial morphogenesis","volume":"13","author":"Levayer","year":"2011","journal-title":"Nat. Cell Biol."},{"key":"2023072308070869600_bib33","doi-asserted-by":"publisher","first-page":"4006","DOI":"10.1242\/dev.111310","article-title":"Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos","volume":"141","author":"Lye","year":"2014","journal-title":"Development."},{"key":"2023072308070869600_bib34","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1016\/j.devcel.2006.04.002","article-title":"Dynasore, a cell-permeable inhibitor of dynamin","volume":"10","author":"Macia","year":"2006","journal-title":"Dev. Cell."},{"key":"2023072308070869600_bib35","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1242\/dev.02197","article-title":"The endocytic pathway and formation of the Wingless morphogen gradient","volume":"133","author":"Marois","year":"2006","journal-title":"Development."},{"key":"2023072308070869600_bib36","doi-asserted-by":"publisher","first-page":"3021","DOI":"10.1242\/dev.01253","article-title":"Parallels between tissue repair and embryo morphogenesis","volume":"131","author":"Martin","year":"2004","journal-title":"Development."},{"key":"2023072308070869600_bib37","doi-asserted-by":"publisher","first-page":"e18729","DOI":"10.1371\/journal.pone.0018729","article-title":"Endocytic and recycling endosomes modulate cell shape changes and tissue behaviour during morphogenesis in Drosophila.","volume":"6","author":"Mateus","year":"2011","journal-title":"PLoS ONE."},{"key":"2023072308070869600_bib38","doi-asserted-by":"publisher","author":"Matsubayashi","year":"2013","DOI":"10.1002\/9780470015902.a0021306"},{"key":"2023072308070869600_bib39","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1242\/jcs.080853","article-title":"\u2018White wave\u2019 analysis of epithelial scratch wound healing reveals how cells mobilise back from the leading edge in a myosin-II-dependent fashion","volume":"124","author":"Matsubayashi","year":"2011","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib40","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1038\/nrm2406","article-title":"Filopodia: molecular architecture and cellular functions","volume":"9","author":"Mattila","year":"2008","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2023072308070869600_bib41","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.ceb.2004.06.005","article-title":"Not just a sink: endosomes in control of signal transduction","volume":"16","author":"Miaczynska","year":"2004","journal-title":"Curr. Opin. Cell Biol."},{"key":"2023072308070869600_bib42","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1242\/dev.014001","article-title":"Dynamic analysis of filopodial interactions during the zippering phase of Drosophila dorsal closure","volume":"135","author":"Millard","year":"2008","journal-title":"Development."},{"key":"2023072308070869600_bib43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1042\/BJ20040176","article-title":"Signalling to actin assembly via the WASP (Wiskott-Aldrich syndrome protein)-family proteins and the Arp2\/3 complex","volume":"380","author":"Millard","year":"2004","journal-title":"Biochem. J."},{"key":"2023072308070869600_bib44","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1083\/jcb.200211083","article-title":"Drosophila p120catenin plays a supporting role in cell adhesion but is not an essential adherens junction component","volume":"160","author":"Myster","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib45","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1242\/jcs.114.3.493","article-title":"Real-time imaging of cell-cell adherens junctions reveals that Drosophila mesoderm invagination begins with two phases of apical constriction of cells","volume":"114","author":"Oda","year":"2001","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib46","doi-asserted-by":"publisher","first-page":"4201","DOI":"10.1242\/jcs.150607","article-title":"Tricellulin regulates junctional tension of epithelial cells at tricellular contacts through Cdc42","volume":"127","author":"Oda","year":"2014","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0074-7696(08)61608-3","article-title":"Role of Rab GTPases in membrane traffic","volume":"176","author":"Olkkonen","year":"1997","journal-title":"Int. Rev. Cytol."},{"key":"2023072308070869600_bib48","doi-asserted-by":"publisher","first-page":"800","DOI":"10.1242\/dev.089557","article-title":"Par3\/Bazooka and phosphoinositides regulate actin protrusion formation during Drosophila dorsal closure and wound healing","volume":"140","author":"Pickering","year":"2013","journal-title":"Development."},{"key":"2023072308070869600_bib49","first-page":"F416","article-title":"An osmotically tolerant inner medullary collecting duct cell line from an SV40 transgenic mouse","volume":"265","author":"Rauchman","year":"1993","journal-title":"Am. J. Physiol."},{"key":"2023072308070869600_bib50","doi-asserted-by":"publisher","first-page":"1814","DOI":"10.1242\/dev.107045","article-title":"Recapitulation of morphogenetic cell shape changes enables wound re-epithelialisation","volume":"141","author":"Razzell","year":"2014","journal-title":"Development."},{"key":"2023072308070869600_bib51","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1242\/bio.20149936","article-title":"Dynamin regulates metaphase furrow formation and plasma membrane compartmentalization in the syncytial Drosophila embryo","volume":"4","author":"Rikhy","year":"2015","journal-title":"Biol. Open."},{"key":"2023072308070869600_bib52","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1016\/j.yexcr.2008.09.021","article-title":"Signaling from endosomes: location makes a difference","volume":"315","author":"Sadowski","year":"2009","journal-title":"Exp. Cell Res."},{"key":"2023072308070869600_bib53","doi-asserted-by":"publisher","first-page":"49031","DOI":"10.1074\/jbc.M308104200","article-title":"Tuba, a novel protein containing bin\/amphiphysin\/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeleton","volume":"278","author":"Salazar","year":"2003","journal-title":"J. Biol. Chem."},{"key":"2023072308070869600_bib54","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1016\/j.ydbio.2007.01.015","article-title":"The Wiskott-Aldrich syndrome protein (WASP) is essential for myoblast fusion in Drosophila.","volume":"304","author":"Sch\u00e4fer","year":"2007","journal-title":"Dev. Biol."},{"key":"2023072308070869600_bib55","doi-asserted-by":"publisher","first-page":"991","DOI":"10.1083\/jcb.200303192","article-title":"Gliotactin, a novel marker of tricellular junctions, is necessary for septate junction development in Drosophila.","volume":"161","author":"Schulte","year":"2003","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib56","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1038\/embor.2009.102","article-title":"Epigenetic reprogramming during wound healing: loss of polycomb-mediated silencing may enable upregulation of repair genes","volume":"10","author":"Shaw","year":"2009","journal-title":"EMBO Rep."},{"key":"2023072308070869600_bib57","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.1242\/jcs.00676","article-title":"An essential role of Rab5 in uniformity of synaptic vesicle size","volume":"116","author":"Shimizu","year":"2003","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib58","doi-asserted-by":"publisher","first-page":"1829","DOI":"10.1242\/jcs.143016","article-title":"Src kinases and ERK activate distinct responses to Stitcher receptor tyrosine kinase signaling during wound healing in Drosophila","volume":"127","author":"Tsarouhas","year":"2014","journal-title":"J. Cell Sci."},{"key":"2023072308070869600_bib59","doi-asserted-by":"publisher","first-page":"890","DOI":"10.1038\/ncb1898","article-title":"The tyrosine kinase Stitcher activates Grainy head and epidermal wound healing in Drosophila","volume":"11","author":"Wang","year":"2009","journal-title":"Nat. Cell Biol."},{"key":"2023072308070869600_bib60","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1038\/ncb875","article-title":"Wound healing recapitulates morphogenesis in Drosophila embryos","volume":"4","author":"Wood","year":"2002","journal-title":"Nat. Cell Biol."},{"key":"2023072308070869600_bib61","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/j.bpj.2009.10.037","article-title":"Quantitative determination of spatial protein-protein correlations in fluorescence confocal microscopy","volume":"98","author":"Wu","year":"2010","journal-title":"Biophys. J."},{"key":"2023072308070869600_bib62","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1083\/jcb.200109057","article-title":"SCAR is a primary regulator of Arp2\/3-dependent morphological events in Drosophila.","volume":"156","author":"Zallen","year":"2002","journal-title":"J. Cell Biol."},{"key":"2023072308070869600_bib63","doi-asserted-by":"publisher","first-page":"2901","DOI":"10.1242\/dev.106898","article-title":"Automated multidimensional image analysis reveals a role for Abl in embryonic wound repair","volume":"141","author":"Zulueta-Coarasa","year":"2014","journal-title":"Development."}],"updated-by":[{"DOI":"10.1083\/jcb.20141103707282015c","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T00:00:00Z","timestamp":1439164800000}}],"container-title":["Journal of Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/210\/3\/419\/1592171\/jcb_201411037.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/rupress.org\/jcb\/article-pdf\/210\/3\/419\/1592171\/jcb_201411037.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,23]],"date-time":"2023-07-23T04:08:08Z","timestamp":1690085288000},"score":1,"resource":{"primary":{"URL":"https:\/\/rupress.org\/jcb\/article\/210\/3\/419\/38191\/Endocytosis-dependent-coordination-of-multiple"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,27]]},"references-count":63,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,8,3]]}},"URL":"https:\/\/doi.org\/10.1083\/jcb.201411037","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725678847.793512075","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.725678847.793509414","asserted-by":"object"}]},"ISSN":["0021-9525","1540-8140"],"issn-type":[{"value":"0021-9525","type":"print"},{"value":"1540-8140","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,27]]}}}