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Furthermore, PI(3,5)P2 competed with actin filaments for binding to cortactin, thereby antagonizing cortactin activity. These findings suggest that PI(3,5)P2 formation on endosomes may remove cortactin from endosome-associated branched actin. Indeed, inhibition of PI(3,5)P2 production led to cortactin accumulation and actin stabilization on Rab7+ endosomes. Conversely, inhibition of Arp2\/3 complex activity greatly reduced cortactin localization to late endosomes. Knockdown of cortactin reversed PI(3,5)P2-inhibitor\u2013induced actin accumulation and stabilization on endosomes. These data suggest a model in which PI(3,5)P2 binding removes cortactin from late endosomal branched actin networks and thereby promotes net actin turnover.<\/jats:p>","DOI":"10.1083\/jcb.201412127","type":"journal-article","created":{"date-parts":[[2015,8,31]],"date-time":"2015-08-31T13:23:13Z","timestamp":1441027393000},"page":"753-769","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":89,"title":["PI(3,5)P2 controls endosomal branched actin dynamics by regulating cortactin\u2013actin interactions"],"prefix":"10.1083","volume":"210","author":[{"given":"Nan Hyung","family":"Hong","sequence":"first","affiliation":[{"name":"Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aidong","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Vanderbilt University Medical Center, Nashville, TN 37232 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alissa M.","family":"Weaver","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, TN 37232 1"},{"name":"Department of Cell and Developmental Biology, Vanderbilt University Medical Center, Nashville, TN 37232 3"},{"name":"Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232 4"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,8,31]]},"reference":[{"key":"2023072308070423800_bib1","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1038\/nature04397","article-title":"Organelle identity and the signposts for membrane traffic","volume":"438","author":"Behnia","year":"2005","journal-title":"Nature."},{"key":"2023072308070423800_bib2","first-page":"227","article-title":"Analysis of cellular phosphoinositides and phosphoinositols by extraction and simple analytical procedures","volume":"27","author":"Bird","year":"1994","journal-title":"Methods Mol. 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