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We also use CRISPR\/Cas9 genome editing in primary human basal cells differentiating into organoids and mucociliary epithelium in vitro. Loss of Grhl2 inhibits organoid morphogenesis and the differentiation of ciliated cells and reduces the expression of both notch and ciliogenesis genes (Mcidas, Rfx2, and Myb) with distinct Grhl2 regulatory sites. The genome editing of other putative target genes reveals roles for zinc finger transcription factor Znf750 and small membrane adhesion glycoprotein in promoting ciliogenesis and barrier function as part of a network of genes coordinately regulated by Grhl2.<\/jats:p>","DOI":"10.1083\/jcb.201506014","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T01:35:27Z","timestamp":1446514527000},"page":"669-682","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":106,"title":["GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells"],"prefix":"10.1083","volume":"211","author":[{"given":"Xia","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aman S.","family":"Bali","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott H.","family":"Randell","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 2"},{"name":"Cystic Fibrosis and Pulmonary Diseases Research and Treatment Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigid L.M.","family":"Hogan","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,11,2]]},"reference":[{"key":"2023072314483670200_bib1","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.ceb.2014.06.013","article-title":"Modeling human development in 3D culture","volume":"31","author":"Ader","year":"2014","journal-title":"Curr. 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