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Apical exocytosis of NHE3, CFTR (cystic fibrosis transmembrane conductance regulator), and GLUT5 required an interaction cascade of Rab11, Myo5B, Slp4a, Munc18-2, and Vamp7 with Stx3, which cooperate in the final steps of this selective apical traffic pathway. The brush border enzymes DPPIV and sucrase-isomaltase still correctly localize at the apical plasma membrane independent of this pathway. Hence, our work demonstrates how Myo5B, Stx3, Slp4a, Vamp7, Munc18-2, and Rab8\/11 cooperate during selective apical cargo trafficking and exocytosis in epithelial cells and thereby provides further insight into MVID pathophysiology.<\/jats:p>","DOI":"10.1083\/jcb.201506112","type":"journal-article","created":{"date-parts":[[2015,11,9]],"date-time":"2015-11-09T18:58:02Z","timestamp":1447095482000},"page":"587-604","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":108,"title":["Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3"],"prefix":"10.1083","volume":"211","author":[{"given":"Georg F.","family":"Vogel","sequence":"first","affiliation":[{"name":"Division of Cell Biology, Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria 1"},{"name":"Division of Histology and Embryology, Medical University of Innsbruck, 6020 Innsbruck, Austria 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina M.C.","family":"Klee","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria 1"},{"name":"Institute of Molecular Biology, University of Innsbruck, 6020 Innsbruck, Austria 4"},{"name":"Center for Molecular Biosciences Innsbruck, University of Innsbruck, 6020 Innsbruck, Austria 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas R.","family":"Janecke","sequence":"additional","affiliation":[{"name":"Department of Paediatrics I, Medical University of Innsbruck, 6020 Innsbruck, Austria 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Paediatrics I, Medical University of Innsbruck, 6020 Innsbruck, Austria 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Hess","sequence":"additional","affiliation":[{"name":"Division of Histology and Embryology, Medical University of Innsbruck, 6020 Innsbruck, Austria 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukas A.","family":"Huber","sequence":"additional","affiliation":[{"name":"Division of Cell Biology, Biocenter, Medical University of Innsbruck, 6020 Innsbruck, Austria 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,11,9]]},"reference":[{"key":"2023072314490439900_bib1","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1083\/jcb.201310114","article-title":"Coordinated binding of Vps4 to ESCRT-III drives membrane neck constriction during MVB vesicle formation","volume":"205","author":"Adell","year":"2014","journal-title":"J. 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