{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T16:29:18Z","timestamp":1782577758063,"version":"3.54.5"},"reference-count":42,"publisher":"Rockefeller University Press","issue":"4","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,11,23]]},"abstract":"<jats:p>Cell\u2013cell recognition guides the assembly of the vertebrate brain during development. \u03b4-Protocadherins comprise a family of neural adhesion molecules that are differentially expressed and have been implicated in a range of neurodevelopmental disorders. Here we show that the expression of \u03b4-protocadherins partitions the zebrafish optic tectum into radial columns of neurons. Using in vivo two-photon imaging of bacterial artificial chromosome transgenic zebrafish, we show that pcdh19 is expressed in discrete columns of neurons, and that these columnar modules are derived from proliferative pcdh19+ neuroepithelial precursors. Elimination of pcdh19 results in both a disruption of columnar organization and defects in visually guided behaviors. These results reveal a fundamental mechanism for organizing the developing nervous system: subdivision of the early neuroepithelium into precursors with distinct molecular identities guides the autonomous development of parallel neuronal units, organizing neural circuit formation and behavior.<\/jats:p>","DOI":"10.1083\/jcb.201507108","type":"journal-article","created":{"date-parts":[[2015,11,23]],"date-time":"2015-11-23T19:09:34Z","timestamp":1448305774000},"page":"807-814","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":75,"title":["Protocadherins control the modular assembly of neuronal columns in the zebrafish optic tectum"],"prefix":"10.1083","volume":"211","author":[{"given":"Sharon R.","family":"Cooper","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 1"},{"name":"Molecular, Cellular, and Developmental Biology Graduate Program, Ohio State University, Columbus, OH 43210 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle R.","family":"Emond","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phan Q.","family":"Duy","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon G.","family":"Liebau","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc A.","family":"Wolman","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of Wisconsin\u2013Madison, Madison, WI 53706 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Jontes","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Ohio State University Wexner Medical Center, Columbus, OH 43210 1"},{"name":"Molecular, Cellular, and Developmental Biology Graduate Program, Ohio State University, Columbus, OH 43210 2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,11,23]]},"reference":[{"key":"2023072315051042100_bib1","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1038\/nature11537","article-title":"In vivo genome editing using a high-efficiency TALEN system","volume":"491","author":"Bedell","year":"2012","journal-title":"Nature."},{"key":"2023072315051042100_bib2","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1007\/s00427-009-0288-6","article-title":"Cloning and characterization of zebrafish protocadherin-17","volume":"219","author":"Biswas","year":"2009","journal-title":"Dev. 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