{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:24:59Z","timestamp":1783689899593,"version":"3.55.0"},"reference-count":56,"publisher":"Rockefeller University Press","issue":"5","content-domain":{"domain":["rupress.org"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,12,7]]},"abstract":"<jats:p>The interaction between centrosomes and mitotic spindle poles is important for efficient spindle formation, orientation, and cell polarity. However, our understanding of the dynamics of this relationship and implications for tissue homeostasis remains poorly understood. Here we report that Drosophila melanogaster calmodulin (CaM) regulates the ability of the microcephaly-associated protein, abnormal spindle (Asp), to cross-link spindle microtubules. Both proteins colocalize on spindles and move toward spindle poles, suggesting that they form a complex. Our binding and structure\u2013function analysis support this hypothesis. Disruption of the Asp\u2013CaM interaction alone leads to unfocused spindle poles and centrosome detachment. This behavior leads to randomly inherited centrosomes after neuroblast division. We further show that spindle polarity is maintained in neuroblasts despite centrosome detachment, with the poles remaining stably associated with the cell cortex. Finally, we provide evidence that CaM is required for Asp\u2019s spindle function; however, it is completely dispensable for Asp\u2019s role in microcephaly suppression.<\/jats:p>","DOI":"10.1083\/jcb.201509054","type":"journal-article","created":{"date-parts":[[2015,12,1]],"date-time":"2015-12-01T03:38:39Z","timestamp":1448941119000},"page":"987-998","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":46,"title":["An Asp\u2013CaM complex is required for centrosome\u2013pole cohesion and centrosome inheritance in neural stem cells"],"prefix":"10.1083","volume":"211","author":[{"given":"Todd","family":"Schoborg","sequence":"first","affiliation":[{"name":"Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allison L.","family":"Zajac","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carey J.","family":"Fagerstrom","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo X.","family":"Guillen","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser M.","family":"Rusan","sequence":"additional","affiliation":[{"name":"Cell Biology and Physiology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2015,11,30]]},"reference":[{"key":"2023072309450291700_bib1","doi-asserted-by":"publisher","first-page":"1375","DOI":"10.1016\/j.cell.2006.05.025","article-title":"Flies without centrioles","volume":"125","author":"Basto","year":"2006","journal-title":"Cell."},{"key":"2023072309450291700_bib2","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1038\/ng995","article-title":"ASPM is a major determinant of cerebral cortical size","volume":"32","author":"Bond","year":"2002","journal-title":"Nat. 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