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The major DNA repair pathway removing DNA lesions caused by exposure to UV light is nucleotide excision repair (NER). We have previously demonstrated that the endoribonuclease DICER facilitates chromatin decondensation during lesion recognition in the global-genomic branch of NER. Here, we report that DICER mediates the recruitment of the methyltransferase MMSET to the DNA damage site. We show that MMSET is required for efficient NER and that it catalyzes the dimethylation of histone H4 at lysine 20 (H4K20me2). H4K20me2 at DNA damage sites facilitates the recruitment of the NER factor XPA. Our work thus provides evidence for an H4K20me2-dependent mechanism of XPA recruitment during lesion recognition in the global-genomic branch of NER.<\/jats:p>","DOI":"10.1083\/jcb.201704028","type":"journal-article","created":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T18:40:52Z","timestamp":1513104052000},"page":"527-540","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["DICER- and MMSET-catalyzed H4K20me2 recruits the nucleotide excision repair factor XPA to DNA damage sites"],"prefix":"10.1083","volume":"217","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3837-269X","authenticated-orcid":true,"given":"Shalaka","family":"Chitale","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Epigenetics, Institute of Molecular Biology, Mainz, Germany 1"},{"name":"Faculty of Biology, Johannes Gutenberg University, Mainz, Germany 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7711-0350","authenticated-orcid":true,"given":"Holger","family":"Richly","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Epigenetics, Institute of Molecular Biology, Mainz, Germany 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2017,12,12]]},"reference":[{"key":"2023072309385083200_bib1","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1101\/gad.373706","article-title":"DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A","volume":"20","author":"Bergink","year":"2006","journal-title":"Genes Dev."},{"key":"2023072309385083200_bib2","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1016\/j.cell.2006.10.043","article-title":"Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair","volume":"127","author":"Botuyan","year":"2006","journal-title":"Cell."},{"key":"2023072309385083200_bib3","doi-asserted-by":"publisher","first-page":"5901","DOI":"10.1093\/nar\/gkx261","article-title":"DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair","volume":"45","author":"Chitale","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"2023072309385083200_bib4","doi-asserted-by":"publisher","first-page":"30870","DOI":"10.18632\/oncotarget.16142","article-title":"Nuclear organization of nucleotide excision repair is mediated by RING1B dependent H2A-ubiquitylation","volume":"8","author":"Chitale","year":"2017","journal-title":"Oncotarget."},{"key":"2023072309385083200_bib5","doi-asserted-by":"publisher","first-page":"39937","DOI":"10.18632\/oncotarget.17773","article-title":"Shaping chromatin with DICER","volume":"8","author":"Chitale","year":"2017","journal-title":"Oncotarget."},{"key":"2023072309385083200_bib6","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1101\/gad.13.7.768","article-title":"Molecular mechanism of nucleotide excision repair","volume":"13","author":"de Laat","year":"1999","journal-title":"Genes Dev."},{"key":"2023072309385083200_bib7","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1006\/scbi.1996.0031","article-title":"Xpa knockout mice","volume":"7","author":"de Vries","year":"1996","journal-title":"Semin. 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