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Each of these actin structures relies on heterodimeric capping protein (CAPZ), which blocks actin polymerization at barbed ends. Because dimension control of the inner ear\u2019s stereocilia is particularly precise, we studied the CAPZB subunit in hair cells. CAPZB, present at \u223c100 copies per stereocilium, concentrated at stereocilia tips as hair cell development progressed, similar to the CAPZB-interacting protein TWF2. We deleted Capzb specifically in hair cells using Atoh1-Cre, which eliminated auditory and vestibular function. Capzb-null stereocilia initially developed normally but later shortened and disappeared; surprisingly, stereocilia width decreased concomitantly with length. CAPZB2 expressed by in utero electroporation prevented normal elongation of vestibular stereocilia and irregularly widened them. Together, these results suggest that capping protein participates in stereocilia widening by preventing newly elongating actin filaments from depolymerizing.<\/jats:p>","DOI":"10.1083\/jcb.201704171","type":"journal-article","created":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T14:10:11Z","timestamp":1505225411000},"page":"3861-3881","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":58,"title":["Heterodimeric capping protein is required for stereocilia length and width regulation"],"prefix":"10.1083","volume":"216","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9516-5163","authenticated-orcid":true,"given":"Matthew R.","family":"Avenarius","sequence":"first","affiliation":[{"name":"Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3015-4081","authenticated-orcid":true,"given":"Jocelyn F.","family":"Krey","sequence":"additional","affiliation":[{"name":"Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8145-4228","authenticated-orcid":true,"given":"Rachel A.","family":"Dumont","sequence":"additional","affiliation":[{"name":"Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8451-6837","authenticated-orcid":true,"given":"Clive P.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor B.","family":"Benson","sequence":"additional","affiliation":[{"name":"Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4652-2002","authenticated-orcid":true,"given":"Sarath","family":"Vijayakumar","sequence":"additional","affiliation":[{"name":"Department of Special Education and Communication Disorders, University of Nebraska-Lincoln, Lincoln, NE 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9619-4190","authenticated-orcid":true,"given":"Christopher L.","family":"Cunningham","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Johns Hopkins University, Baltimore, MD 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah I.","family":"Scheffer","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P.","family":"Corey","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Harvard Medical School, Boston, MA 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Johns Hopkins University, Baltimore, MD 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5018-3659","authenticated-orcid":true,"given":"Sherri M.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Special Education and Communication Disorders, University of Nebraska-Lincoln, Lincoln, NE 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9787-5860","authenticated-orcid":true,"given":"Peter G.","family":"Barr-Gillespie","sequence":"additional","affiliation":[{"name":"Oregon Hearing Research Center and Vollum Institute, Oregon Health and Science University, Portland, OR 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2017,9,12]]},"reference":[{"key":"2023072317594294200_bib1","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/S0092-8674(00)80181-6","article-title":"RAG1 and RAG2 form a stable postcleavage synaptic complex with DNA containing signal ends in V(D)J recombination","volume":"89","author":"Agrawal","year":"1997","journal-title":"Cell."},{"key":"2023072317594294200_bib2","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1083\/jcb.119.5.1151","article-title":"Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast","volume":"119","author":"Amatruda","year":"1992","journal-title":"J. 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