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Lysosomal sorting is mediated by interaction of ubiquitylated transmembrane proteins with the endosomal sorting complex required for transport (ESCRT) machinery. In this study, we uncover an alternative role for the ESCRT-0 component hepatocyte growth factor\u2013regulated tyrosine kinase substrate (HRS) in promoting the constitutive recycling of transmembrane proteins. We find that endosomal localization of the actin nucleating factor Wiscott-Aldrich syndrome protein and SCAR homologue (WASH) requires HRS, which occupies adjacent endosomal subdomains. Depletion of HRS results in defective constitutive recycling of epidermal growth factor receptor and the matrix metalloproteinase MT1\u2013MMP, leading to their accumulation in internal compartments. We show that direct interactions with endosomal actin are required for efficient recycling and use a model system of chimeric transferrin receptor trafficking to show that an actin-binding motif can counteract an ubiquitin signal for lysosomal sorting. Directed receptor recycling is used by cancer cells to achieve invasive migration. Accordingly, abrogating HRS- and actin-dependent MT1-MMP recycling results in defective matrix degradation and invasion of triple-negative breast cancer cells.<\/jats:p>","DOI":"10.1083\/jcb.201710051","type":"journal-article","created":{"date-parts":[[2018,6,11]],"date-time":"2018-06-11T13:10:00Z","timestamp":1528722600000},"page":"2549-2564","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":61,"title":["HRS\u2013WASH axis governs actin-mediated endosomal recycling and cell invasion"],"prefix":"10.1083","volume":"217","author":[{"given":"Ewan","family":"MacDonald","sequence":"first","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5631-2789","authenticated-orcid":true,"given":"Louise","family":"Brown","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Selvais","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Cancer Stem Cell, Dalian Medical University, Dalian, China 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3280-8886","authenticated-orcid":true,"given":"Thomas","family":"Waring","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0534-8914","authenticated-orcid":true,"given":"Daniel","family":"Newman","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Bithell","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1034-6625","authenticated-orcid":true,"given":"Douglas","family":"Grimes","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4735-9814","authenticated-orcid":true,"given":"Sylvie","family":"Urb\u00e9","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3355-9479","authenticated-orcid":true,"given":"Michael J.","family":"Clague","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8394-088X","authenticated-orcid":true,"given":"Tobias","family":"Zech","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, Cellular and Molecular Physiology, University of Liverpool, Liverpool, England, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2018,6,11]]},"reference":[{"key":"2023072309523744400_bib1","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1083\/jcb.125.1.67","article-title":"Receptor-mediated transcytosis of IgA in MDCK cells is via apical recycling endosomes","volume":"125","author":"Apodaca","year":"1994","journal-title":"J. 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