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The directed recruitment of the metalloproteinase MT1-MMP to invadopodia plays a critical role in this invasive process. Here, we provide mechanistic insight into MT1-MMP cytoplasmic tail binding protein 1 (MTCBP-1) with respect to invadopodia formation, matrix remodeling, and invasion by pancreatic tumor cells. MTCBP-1 localizes to invadopodia and interacts with MT1-MMP. We find that this interaction displaces MT1-MMP from invadopodia, thereby attenuating their number and function and reducing the capacity of tumor cells to degrade matrix. Further, we observe an inverse correlation between MTCBP-1 and MT1-MMP expression both in cultured cell lines and human pancreatic tumors. Consistently, MTCBP-1\u2013expressing cells show decreased ability to invade in vitro and metastasize in vivo. These findings implicate MTCBP-1 as an inhibitor of the metastatic process.<\/jats:p>","DOI":"10.1083\/jcb.201802032","type":"journal-article","created":{"date-parts":[[2018,11,28]],"date-time":"2018-11-28T20:49:23Z","timestamp":1543438163000},"page":"317-332","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":41,"title":["Pancreatic tumor cell metastasis is restricted by MT1-MMP binding protein MTCBP-1"],"prefix":"10.1083","volume":"218","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2354-177X","authenticated-orcid":true,"given":"Li","family":"Qiang","sequence":"first","affiliation":[{"name":"Biochemistry and Molecular Biology Program, Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Cao","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8648-9569","authenticated-orcid":true,"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaun G.","family":"Weller","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-9075","authenticated-orcid":true,"given":"Eugene W.","family":"Krueger","sequence":"additional","affiliation":[{"name":"Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Mayo Clinic, Rochester, MN 4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gina L.","family":"Razidlo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 2"},{"name":"Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9830-1885","authenticated-orcid":true,"given":"Mark A.","family":"McNiven","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 2"},{"name":"Center for Basic Research in Digestive Diseases, Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN 3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2018,11,28]]},"reference":[{"key":"2023072315180205700_bib1","volume-title":"Cancer Facts & Figures 2015.","author":"American Cancer Society","year":"2015"},{"key":"2023072315180205700_bib2","volume-title":"Cancer Facts & Figures 2017.","author":"American Cancer Society","year":"2017"},{"key":"2023072315180205700_bib3","doi-asserted-by":"publisher","first-page":"3034","DOI":"10.1158\/0008-5472.CAN-05-2177","article-title":"Dynamic interactions of cortactin and membrane type 1 matrix metalloproteinase at invadopodia: defining the stages of invadopodia formation and function","volume":"66","author":"Artym","year":"2006","journal-title":"Cancer Res."},{"key":"2023072315180205700_bib4","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1083\/jcb.200107107","article-title":"Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior","volume":"155","author":"Ballestrem","year":"2001","journal-title":"J. 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