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Defects in the assembly of a collagen matrix lead to pathologies including fibrosis and osteogenesis imperfecta. Owing to the size of fibril-forming procollagen molecules it is assumed that they are transported from the endoplasmic reticulum to the Golgi in specialized large COPII-dependent carriers. Here, analyzing endogenous procollagen and a new engineered GFP-tagged form, we show that transport to the Golgi occurs in the absence of large (&amp;gt;350 nm) carriers. Large GFP-positive structures were observed occasionally, but these were nondynamic, are not COPII positive, and are labeled with markers of the ER. We propose a short-loop model of COPII-dependent ER-to-Golgi traffic that, while consistent with models of ERGIC-dependent expansion of COPII carriers, does not invoke long-range trafficking of large vesicular structures. Our findings provide an important insight into the process of procollagen trafficking and reveal a short-loop pathway from the ER to the Golgi, without the use of large carriers.<\/jats:p>","DOI":"10.1083\/jcb.201806035","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T14:25:11Z","timestamp":1545834311000},"page":"929-948","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":118,"title":["ER-to-Golgi trafficking of procollagen in the absence of large carriers"],"prefix":"10.1083","volume":"218","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7709-2597","authenticated-orcid":true,"given":"Janine","family":"McCaughey","sequence":"first","affiliation":[{"name":"Cell Biology Laboratories, School of Biochemistry, Faculty of Life Sciences, University of Bristol, Bristol, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8967-7277","authenticated-orcid":true,"given":"Nicola L.","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Cell Biology Laboratories, School of Biochemistry, Faculty of Life Sciences, University of Bristol, Bristol, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Cross","sequence":"additional","affiliation":[{"name":"Wolfson Bioimaging Facility, Faculty of Biomedical Sciences, University of Bristol, Bristol, UK 2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5297-3240","authenticated-orcid":true,"given":"David J.","family":"Stephens","sequence":"additional","affiliation":[{"name":"Cell Biology Laboratories, School of Biochemistry, Faculty of Life Sciences, University of Bristol, Bristol, UK 1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"291","published-online":{"date-parts":[[2018,12,26]]},"reference":[{"key":"2023072310090446500_bib1","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1083\/jcb.131.4.875","article-title":"Sequential coupling between COPII and COPI vesicle coats in endoplasmic reticulum to Golgi transport","volume":"131","author":"Aridor","year":"1995","journal-title":"J. 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