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The damage includes severe morphological changes with destabilization of SDs and their replacement by junctional complexes between abnormally broadened foot processes. In Drosophila melanogaster, SDs are present in nephrocytes, which filter the fly's hemolymph. Here, we show that a specific isoform of Polychaetoid\/ZO-1, Pyd-P, is essential for Drosophila SDs, since, in pyd mutants devoid of Pyd-P, SDs do not form and the SD component Dumbfounded accumulates at ectopic septate-like junctions between abnormally aggregated nephrocytes. Reintroduction of Pyd-P leads to junctional remodeling and their progressive normalization toward SDs. This transition requires the coiled-coil domain of Pyd-P and implies formation of nonclathrin vesicles containing SD components and their trafficking to the nephrocyte external membrane, where SDs assemble. Analyses in zebrafish suggest a conserved role for Tjp1a\/ZO-1 in promoting junctional remodeling in podocytes.<\/jats:p>","DOI":"10.1083\/jcb.201810171","type":"journal-article","created":{"date-parts":[[2019,6,6]],"date-time":"2019-06-06T18:04:08Z","timestamp":1559844248000},"page":"2294-2308","update-policy":"https:\/\/doi.org\/10.1083\/jcb.crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["A specific isoform of Pyd\/ZO-1 mediates junctional remodeling and formation of slit diaphragms"],"prefix":"10.1083","volume":"218","author":[{"given":"Marta","family":"Carrasco-Rando","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas and Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Prieto-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas and Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joaquim","family":"Culi","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas and Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio S.","family":"Tutor","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas and Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9024-1400","authenticated-orcid":true,"given":"Mar","family":"Ruiz-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular Severo Ochoa, Consejo Superior de Investigaciones Cient\u00edficas and Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"291","published-online":{"date-parts":[[2019,6,6]]},"reference":[{"key":"2023072315470058300_bib1","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1007\/BF01252944","article-title":"The ultrastructure of the wreath cells of Drosophila melanogaster larvae","volume":"63","author":"Aggarwal","year":"1967","journal-title":"Protoplasma."},{"key":"2023072315470058300_bib2","doi-asserted-by":"publisher","first-page":"C918","DOI":"10.1152\/ajpcell.1993.264.4.C918","article-title":"Two classes of tight junctions are revealed by ZO-1 isoforms","volume":"264","author":"Balda","year":"1993","journal-title":"Am. 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